Fc gamma RIIB/CD32b 蛋白, Mouse (HEK293, His)

Fc gamma RIIB/CD32b protein is a low-affinity receptor for immunoglobulin gamma and is involved in a variety of effector and regulatory functions. When co-aggregated with BCR, TCR and Fc receptors, it promotes endocytosis of soluble immune complexes (IIB2), modulates antibody production, and downregulates B-cell, T-cell and mast cell activation. Fc gamma RIIB/CD32b Protein, Mouse (HEK293, His) is the recombinant mouse-derived Fc gamma RIIB/CD32b protein, expressed by HEK293 , with C-6*His labeled tag.

Fc gamma RIIB/CD32b 蛋白作为复合免疫球蛋白 gamma Fc 区的受体，具有低亲和力。它在各种效应器和调节功能中发挥作用，包括吞噬循环中的抗原-抗体复合物以及调节 B 细胞产生抗体。异构体 IIB1 和异构体 IIB1' 形成帽，但不介导内吞作用或吞噬作用，而异构体 IIB2 可以通过网格蛋白包被的凹坑促进可溶性免疫复合物的内吞作用。当与 AG 的 B 细胞受体 (BCR)、AG 的 T 细胞受体 (TCR) 和 Fc 受体共聚集时，亚型 IIB1 和亚型 IIB2 均可下调 B 细胞、T 细胞和肥大细胞的活化，分别。Fc gamma RIIB/CD32b 与 FGR 和 LYN 相互作用（通过相似性）。

Loaded Recombinant Mouse CD32b on HIS1K Biosensor, can bind Recombinant Mouse IgG1 Fc with an affinity constant of 64.9 nM as determined in BLI assay.

Mouse

HEK293

C-6*His

P08101-1 (T30-P210)

14130  [NCBI]

Extracellular Domain

THDLPKAVVKLEPPWIQVLKEDTVTLTCEGTHNPGNSSTQWFHNGRSIRSQVQASYTFKATVNDSGEYRCQMEQTRLSDPVDLGVISDWLLLQTPQLVFLEGETITLRCHSWRNKLLNRISFFHNEKSVRYHHYSSNFSIPKANHSHSGDYYCKGSLGRTLHQSKPVTITVQGPKSSRSLP

Approximately 31 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.