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  4. CD38
  5. CD38 蛋白, Human (HEK293, Fc)

CD38 蛋白, Human (HEK293, Fc)

目录号: HY-P70022
COA 产品使用指南

CD38 蛋白在细胞信号传导中发挥着关键作用,能够熟练地合成环状 ADP-核糖 (cADPR),作为葡萄糖诱导的胰岛素分泌的关键第二信使。它还促进钙动员剂烟酸-腺嘌呤二核苷酸磷酸 (NAADP+) 的合成。CD38 蛋白, Human (HEK293, Fc) 是重组的 CD38 蛋白,由 HEK293 表达,带有 C-hFc 标签。CD38 蛋白, Human (HEK293, Fc) 全长 258 个氨基酸,分子量为 75-90 kDa。

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5 μg ¥750 In-stock
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50 μg ¥3500 In-stock
100 μg ¥6000 In-stock
500 μg ¥13000 In-stock
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描述

The CD38 protein plays a key role in cell signaling, proficiently synthesizing cyclic ADP-ribose (cADPR) as a critical second messenger for glucose-induced insulin secretion. It also promotes the synthesis of the calcium mobilizer niacin-adenine dinucleotide phosphate (NAADP+). CD38 Protein, Human (HEK293, Fc) is the recombinant human-derived CD38 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD38 Protein, Human (HEK293, Fc) is 258 a.a., with molecular weight of 75-90 kDa.

研究背景

CD38 蛋白在细胞信号传导中发挥着关键作用,是环状 ADP-核糖 (cADPR) 的熟练合成者,而 cADPR 是公认的对葡萄糖诱导的胰岛素分泌至关重要的第二信使。此外,它还促进钙动员剂烟酸-腺嘌呤二核苷酸磷酸、NAADP(+) 的合成,NAADP(+) 源自 2'-磷酸-cADPR 和烟酸,以及 NADP(+) 和烟酸。在 pH 5.0 和 pH 7.4 下工作时,CD38 蛋白优先将 2'-磷酸-cADPR 转化为 NAADP(+),同时选择性地将 NADP(+) 裂解为 cADPR 和 ADPRP,而不是生成 NADDP(+)。值得注意的是,它还展示了 cADPR 水解酶活性,突出了其在这些关键信号分子动态调节中的多方面作用。这些活性的整合强调了 CD38 蛋白在调节细胞反应中的重要性,特别是在胰岛素分泌和钙动员的背景下。

生物活性

Immobilized Human CD38 at 2 μg/mL (100 μL/well) can bind Anti-CD38 Antibody. The ED50 for this effect is 11.99 ng/mL, corresponding to a specific activity is 8.34×104 units/mg.

  • Immobilized Human CD38 at 2 μg/mL (100 μL/well) can bind Anti-CD38 Antibody, The ED50 for this effect is 11.99 ng/mL,corresponding to a specific activity is 8.34×104units/mg.
种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

P28907 (V43-I300)

基因 ID

952  [NCBI]

蛋白结构
N-term
CD38 (V43-I300)
Accession # P28907
hFc
C-term
同用名
rHuADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, Fc; ADP-ribosyl cyclase 1; cyclic ADP-ribose hydrolase; CD38; T10
氨基酸序列

VPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI

分子量

75-90 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD38 Protein, Human (HEK293, Fc)
目录号:
HY-P70022
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