1. 重组蛋白
  2. CD Antigens
  3. T Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. CD39
  5. CD39 蛋白, Human (sf9, His)

CD39 蛋白, Human (sf9, His)

目录号: HY-P75417
COA 产品使用指南

CD39蛋白是神经系统中的重要调节因子,通过水解ATP和其他核苷酸来协调嘌呤能神经传递。其酶活性可将血小板活化的ADP水解成AMP,从而有效阻止血小板聚集。CD39 蛋白, Human (sf9, His) 是重组的 CD39 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。CD39 蛋白, Human (sf9, His) 全长 441 个氨基酸,分子量约为 ~51.8 kDa。

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重组蛋白定制服务

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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10 μg ¥1480 In-stock
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50 μg ¥4500 In-stock
100 μg   询价  

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描述

The CD39 protein is an important regulator in the nervous system, coordinating purinergic neurotransmission by hydrolyzing ATP and other nucleotides. Its enzymatic ability can hydrolyze platelet-activated ADP into AMP, thereby effectively preventing platelet aggregation. CD39 Protein, Human (sf9, His) is the recombinant human-derived CD39 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of CD39 Protein, Human (sf9, His) is 441 a.a., with molecular weight of ~51.8 kDa.

研究背景

在神经系统中,CD39 蛋白通过水解 ATP 和其他核苷酸,充当嘌呤能神经传递的关键调节剂。其酶活性延伸至预防血小板聚集,因为它能有效地将血小板活化的 ADP 水解为 AMP。值得注意的是,CD39 在水解 ATP 和 ADP 方面表现出同等的能力,强调了其在调节嘌呤能信号通路以及促进神经传递和血小板功能的复杂调节方面的多功能作用。

生物活性

Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP) and the specific activity is >5000 pmol/min/µg.

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P49961 (T38-V478)

基因 ID

953  [NCBI]

蛋白结构
N-term
CD39 (T38-V478)
Accession # P49961
His
C-term
同用名
Ectonucleoside triphosphate diphosphohydrolase 1; NTPDase 1; Ecto-apyrase; CD39; Entpd1
分子量

Approximately 51.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.1.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD39 Protein, Human (sf9, His)
目录号:
HY-P75417
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