1. 重组蛋白
  2. CD Antigens
  3. T Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. CD39
  5. CD39 蛋白, Mouse (His)

CD39 蛋白, Mouse (His)

目录号: HY-P72898
产品使用指南

CD39蛋白在神经系统中表达,通过水解ATP和其他核苷酸来调节嘌呤能神经传递。它还通过将血小板激活的 ADP 水解为 AMP 来防止血小板聚集。CD39 蛋白, Mouse (His) 是重组的 CD39 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。CD39 蛋白, Mouse (His) 全长 441 个氨基酸,分子量约为 ~51 kDa。

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描述

The CD39 protein is expressed in the nervous system and regulates purinergic neurotransmission by hydrolyzing ATP and other nucleotides. It also prevents platelet aggregation by hydrolyzing platelet-activated ADP to AMP. CD39 Protein, Mouse (His) is the recombinant mouse-derived CD39 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of CD39 Protein, Mouse (His) is 441 a.a., with molecular weight of ~51 kDa.

研究背景

CD39 蛋白在神经系统中显着表达,通过水解 ATP 和其他核苷酸,在嘌呤能神经传递的调节中发挥着至关重要的作用。此外,CD39 通过将血小板活化 ADP 水解为 AMP 来防止血小板聚集。值得注意的是,CD39 在水解 ATP 和 ADP 方面表现出同等的能力,强调了其在调节嘌呤能信号通路和促进影响神经传递和血小板功能的调节机制方面的多功能性。CD39 的双重酶活性凸显了其在维持神经系统内嘌呤能信号传导的微妙平衡和更广泛的止血方面的重要性。

种属

Mouse

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P55772 (T38-I478)

基因 ID

12495  [NCBI]

蛋白结构
N-term
CD39 (T38-I478)
Accession # P55772
His
C-term
同用名
Ectonucleoside triphosphate diphosphohydrolase 1; NTPDase 1; Ecto-apyrase; CD39; Entpd1
氨基酸序列

TQNKPLPENVKYGIVLDAGSSHTNLYIYKWPAEKENDTGVVQQLEECQVKGPGISKYAQKTDEIGAYLAECMELSTELIPTSKHHQTPVYLGATAGMRLLRMESEQSADEVLAAVSTSLKSYPFDFQGAKIITGQEEGAYGWITINYLLGRFTQEQSWLSLISDSQKQETFGALDLGGASTQITFVPQNSTIESPENSLQFRLYGEDYTVYTHSFLCYGKDQALWQKLAKDIQVSSGGVLKDPCFNPGYEKVVNVSELYGTPCTKRFEKKLPFDQFRIQGTGDYEQCHQSILELFNNSHCPYSQCAFNGVFLPPLHGSFGAFSAFYFVMDFFKKVAKNSVISQEKMTEITKNFCSKSWEETKTSYPSVKEKYLSEYCFSGAYILSLLQGYNFTDSSWEQIHFMGKIKDSNAGWTLGYMLNLTNMIPAEQPLSPPLPHSTYI

分子量

Approximately 51 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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