CD3E-CD3G Heterodimer 蛋白, Mouse (HEK293, Fc)

The CD3E protein, with predicted functions like SH3 domain binding and protein heterodimerization, plays a crucial role in nervous system development and cell adhesion. It is involved in T cell activation, cytokine production, and signal transduction. Located in various cellular components, its expression is observed in the colon and hemolymphoid system. CD3E, the human ortholog, has implications in immunodeficiency 18, emphasizing its role in immune system functionality. Biased expression occurs in the thymus and spleen, highlighting its importance in immune processes. CD3E-CD3G Heterodimer Protein, Mouse (HEK293, Fc) is a recombinant protein dimer complex containing mouse-derived CD3E-CD3G Heterodimer protein, expressed by HEK293, with C-hFc labeled tag. CD3E-CD3G Heterodimer Protein, Mouse (HEK293, Fc), has molecular weight of ~38-40 kDa.

CD3E蛋白预计具有SH3结构域结合活性、相同蛋白结合活性和蛋白异二聚化活性等多种功能，在神经系统发育和细胞粘附的正向调节中发挥着至关重要的作用。它积极参与多个过程的上游，包括T细胞激活的正向调节、细胞因子产生的正向调节以及信号转导的调节。该蛋白质位于多种细胞成分中，包括树突棘、质膜外侧和免疫突触。作为 α-β T 细胞受体复合物的一部分，在结肠和血淋巴系统中观察到其表达。该基因的人类直系同源物 CD3E 对免疫缺陷 18 具有影响，凸显了其在免疫系统功能中的重要性。胸腺和脾脏等组织中存在明显的偏向表达，强调了其在免疫过程中的作用。

Immobilized Human CD3E-CD3G at 2 μg/mL (100 μL/well) can bind Anti-CD3 Antibody. The ED₅₀ for this effect is 1.792 ng/mL.

• 
  Immobilized Human CD3E-CD3G at 2 μg/mL (100 μL/well) can bind Anti-CD3 Antibody, The ED₅₀ for this effect is 1.792ng/mL.

Mouse

HEK293

C-hFc

NP_031674.1 (D23-D108)&NP_033980.1 (Q23-S116)

12501  [NCBI]&12502  [NCBI]

DAENIEYKVSISGTSVELTCPLDSDENLKWEKNGQELPQKHDKHLVLQDFSEVEDSGYYVCYTPASNKNTYLYLKARVCEYCVEVD
&:
QTNKAKNLVQVDGSRGDGSVLLTCGLTDKTIKWLKDGSIISPLNATKNTWNLGNNAKDPRGTYQCQGAKETSNPLQVYYRMCENCIELNIGTIS

Approximately 38-40 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.