1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory Immune Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins
  4. TNF Superfamily Ligands CD40 Ligand/CD154
  5. CD40 Ligand
  6. CD40L/CD154/TRAP 蛋白, Human (His)

CD40L/CD154/TRAP 蛋白, Human (His)

目录号: HY-P72907
COA 产品使用指南

CD40L (CD154; TRAP) 是 CD40 (TNFRSF5) 的配体,在 T 细胞中产生共刺激信号而发挥上调 IL-4 合成的作用。CD40L 在活化的 CD4+ T 淋巴细胞上特异性表达,参与 NF-κB/MAPK 信号通路。CD40L 还参与 B 细胞的分化、成熟和凋亡。人 CD40L 蛋白可裂解为 2 种亚型,包括膜状肽 (1-261 a.a.) 和可溶性肽 (113-261 a.a.) (作为整合素的配体)。重组人 CD40L/CD154/TRAP 蛋白 (His) 全长 144 个氨基酸 (E108-L261),由 E. coli 细胞表达,带有 C 端 His 标签。

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描述

CD40L (CD154; TRAP) is a ligand to CD40/TNFRSF5, acts function by generating a costimulatory signal that up-regulates IL-4 synthesis[1]. CD40L is specifically expressed on activated CD4+ T-lymphocytes and involves in activation of NF-κB/MAPK pathway[2][3]. CD40L also involves in B cell differentiation, maturation, and apoptosis[4]. CD40L in human, cleaved into 2 chains of membrane form (1-261 a.a.) and soluble form (113-261 a.a.) which serves as a ligand for integrins (ITGA5:ITGB1 and ITGAV:ITGB3). CD40L/CD154/TRAP Protein, Human (His) has a total length of 144 amino acids (E108-L261), is expressed in E. coli cells with C-terminal His-tag.

研究背景

CD40 配体(CD40L;CD154;TRAP)属于肿瘤坏死因子 (TNF) 家族,是 CD40/TNFRSF5 的配体,特异性表达于活化的 CD4+ T 淋巴细胞[1]
CD40L 是 B 细胞上的一种 II 型跨膜蛋白,可触发 B 细胞分化、成熟和凋亡的重要信号[4]
CD40L 通过与 T 细胞交联来发挥作用产生共刺激信号,从而与 TCR/CD3 连接和 CD28 共刺激一起增强 IL4 和 IL10 的产生,并促进干扰素-γ 和 TNF-α[1] 的产生[4]
CD40L 与抗原呈递细胞 (APC) 上的 CD40 结合,通过刺激 I-κB 激酶 (IKK) 激活 TNFR 相关因子 2 和 IKK2 依赖性通路,增加 NF-κB DNA 结合和 p65 核转位。I-κB 激酶的激活导致 c-Jun N 末端激酶强烈激活以及 GST-I-κB 和 GST-p65 磷酸化[2]
CD40L 参与 MAPK强烈抑制 Bcl-6 的途径,诱导 Erk1/2、p38 和 Jnk1/2 磷酸化并激活 NF-κB[3] 介导的 IRF4。
CD40L 还结合并发出信号通过几个整合素,包括 αvβ3α5β1,它们与 CD40L 的三聚体界面结合。CD40L 在免疫反应中发挥重要作用,是炎症的主要靶点[5]
CD40L 广泛存在于不同动物中,而人类中的序列与恒河猴高度相似( 98.08%),但与大鼠和小鼠有很大不同,相似度分别为 77.31% 和 77.69%。人体内的CD40L被切割成膜形式(1-261aa)和可溶性形式(113-261aa)的2条链,而可溶性形式是通过蛋白水解加工从膜形式衍生出来的。血小板中可溶性 CD40L 的释放部分受到 GP IIb/IIIa、肌动蛋白聚合和基质金属蛋白酶 (MMP) 抑制剂敏感途径[6]的调节。

体外研究
(In Vitro)

CD4 Ligand (2.86 ng/mL or 2.9 ng/mL) has been demonstrated to be a mark of cardiovascular risk, while the level (2.86 ng/mL) in who subsequently developed myocardial infarction, stroke, or cardiovascular death (cases) is higher than control group (2.9 ng/mL)[8].

体内研究
(In Vivo)

CD4 Ligand (1 ng/mL; 24 hr) induces endothelial cell vascular cell adhesion molecule-1 (VCAM-1) expression. CD4 Ligand shows costimulation effect with IL-4 against VCAM-1[7].
CD4 Ligand (1 ng/mL; 24 hr) combined with IL-4 enhances IL-6 and inhibits IL-8 production by HUVEC[7].

生物活性

Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μl/well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of ≤0.94 μg/mL determined by ELISA.

种属

Human

表达系统

E. coli

标签

C-His

蛋白编号

P29965 (Q114-L261)

基因 ID

959  [NCBI]

蛋白结构
N-term
CD40L (Q114-L261)
Accession # P29965
His
C-term
中文名
重组人 CD40L/CD154/TRAP 蛋白 (His)
同用名
CD40 ligand; CD40-L; TRAP; CD154; sCD40L; TNFSF5
氨基酸序列

QKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

分子量

Approximately 17.7 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4, 10% Glycerol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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