CD40L/CD154/TRAP 蛋白, Human (P. pastoris, His)

CD40L/CD154/TRAP proteins stimulate T cell proliferation and cytokine production, acting as ligands for integrins and CD40 receptors to activate various cell-dependent effects such as B cell activation and NF-κ-B signaling. CD40L/CD154/TRAP Protein, Human (P. pastoris, His) is the recombinant human-derived CD40L/CD154/TRAP protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of CD40L/CD154/TRAP Protein, Human (P. pastoris, His) is 215 a.a., with molecular weight of 26.1 kDa.

CD40L，也称为 CD154 或 TRAP 蛋白，具有细胞因子的功能，并作为 CD40/TNFRSF5 的配体，协调一系列免疫反应。它在 T 细胞激活中发挥着关键作用，充当有效的共刺激剂，增强 T 细胞增殖和细胞因子的产生，特别是 IL4 和 IL10。在 T 细胞上交联后，CD40L 会产生与 TCR/CD3 连接和 CD28 共刺激协同的共刺激信号。此外，CD40L 还可诱导 NF-kappa-B 的激活，触发 T 细胞中激酶 MAPK8 和 PAK2 的激活，并促进 CD28 异构体 3 的酪氨酸磷酸化。除了 T 细胞调节之外，CD40L 在不存在的情况下还可介导 B 细胞增殖。共刺激并在 IL4 存在的情况下促进 IgE 产生，有助于免疫球蛋白类别转换。此外，它作为整合素的配体，特别是 ITGA5:ITGB1 和 ITGAV:ITGB3，并与 CD40 受体合作激活 CD40-CD40LG 信号传导，发挥细胞类型依赖性效应，例如 B 细胞激活、NF- kappa-B 信号传导和抗凋亡信号传导。

Human

P. pastoris

N-6*His

P29965 (H47-L261)

959  [NCBI]

HRRLDKIEDERNLHEDFVFMKTIQRCNTGERSLSLLNCEEIKSQFEGFVKDIMLNKEETKKENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

31 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.