1. 重组蛋白
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  3. Inhibitory Checkpoint Molecules Stem Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. Integrin Associated Protein/CD47
  5. CD47 蛋白, Human (Biotinylated, HEK293, His-Avi)

CD47 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P78095
COA 产品使用指南

CD47 是一种粘附蛋白,通过 G 蛋白激活调节整合素信号传导,并作为血小板反应蛋白 THBS1 的受体,影响信号转导、心血管稳态、炎症、细胞凋亡、血管生成、自我更新和免疫调节。它调节肺内皮素 EDN1 信号传导,充当血压调节的升压剂,并且对于记忆形成至关重要。CD47 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 CD47 蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。CD47 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 121 个氨基酸,分子量为 45-55 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
20 μg ¥2550 In-stock
50 μg ¥5610 In-stock
100 μg ¥9450 询价
> 100 μg   询价  

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描述

CD47 is an adhesion protein that regulates integrin signaling through G protein activation and serves as a receptor for thrombospondin THBS1, affecting signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, self-renewal and Immunomodulatory. It regulates pulmonary endothelin EDN1 signaling, acts as a pressor in blood pressure regulation, and is critical for memory formation. CD47 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD47 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CD47 Protein, Human (Biotinylated, HEK293, His-Avi) is 121 a.a., with molecular weight of 45-55 kDa.

研究背景

CD47 是一种粘附蛋白,可促进细胞间相互作用,并作为血小板反应蛋白 THBS1 的受体,通过激活异三聚体 G 蛋白来调节整合素信号传导。CD47 参与多种细胞过程,有助于信号转导、心血管稳态、炎症、细胞凋亡、血管生成、细胞自我更新和免疫调节。值得注意的是,它在调节肺内皮素 EDN1 信号传导中发挥作用,并在响应 THBS1 的血压调节中充当升压剂。CD47 对于海马体的记忆形成和突触可塑性至关重要,作为 SIRPA 和 SIRPG 的受体,影响树突状细胞的成熟、细胞因子的产生、细胞间粘附和 T 细胞激活。此外,CD47 正向调节 T 细胞中 FAS 依赖性细胞凋亡并抑制血管生成,从而导致衰老过程中的代谢失调。作为对 THBS1 的反应,CD47 负向调节伤口愈合,抑制干细胞自我更新,并可能在膜运输和/或整合素依赖性信号转导中发挥作用。作为单体,CD47 与 THBS1、SIRPA、FAS/CD95、SIRPG、UBQLN1、UBQLN2 和潜在的纤维蛋白原相互作用,突出了其在细胞和分子途径中的复杂参与。

生物活性

Immobilized Human SIRP alpha, hFc Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Human CD47, His Tag with the EC50 of 0.48-0.83 μg/ml determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q08722 (Q19-P139)

基因 ID

961  [NCBI]

蛋白结构
N-term
CD47 (Q19-P139)
Accession # Q08722
His-Avi
C-term
同用名
CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
氨基酸序列

QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP

分子量

45-55 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD47 Protein, Human (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78095
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