CD47 蛋白, Human (Biotinylated, HEK293, mFc-Avi)

FITC-Labeled CD47, an adhesive protein, modulates integrin signaling through G protein activation and serves as a receptor for thrombospondin THBS1, impacting signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, self-renewal, and immunoregulation. It regulates pulmonary endothelin EDN1 signaling, acts as a pressor agent in blood pressure regulation, and is crucial for memory formation. CD47's interactions with SIRPA, SIRPG, and FAS/CD95 influence dendritic cell maturation, T-cell activation, apoptosis, and angiogenesis. As a monomer, CD47 intricately participates in diverse cellular and molecular pathways, contributing to physiological and pathological processes. CD47 Protein, Human (Biotinylated, HEK293, mFc-Avi) is the recombinant human-derived CD47 protein, expressed by HEK293, with C-mFc, C-Avi labeled tag. The total length of CD47 Protein, Human (Biotinylated, HEK293, mFc-Avi) is 121 a.a., with molecular weight of 60-66 kDa.

CD47 是一种粘附蛋白，可促进细胞间相互作用，并作为血小板反应蛋白 THBS1 的受体，通过激活异三聚体 G 蛋白来调节整合素信号传导。CD47 参与多种细胞过程，有助于信号转导、心血管稳态、炎症、细胞凋亡、血管生成、细胞自我更新和免疫调节。值得注意的是，它在调节肺内皮素 EDN1 信号传导中发挥作用，并在响应 THBS1 的血压调节中充当升压剂。CD47 对于海马体的记忆形成和突触可塑性至关重要，作为 SIRPA 和 SIRPG 的受体，影响树突状细胞的成熟、细胞因子的产生、细胞间粘附和 T 细胞激活。此外，CD47 正向调节 T 细胞中 FAS 依赖性细胞凋亡并抑制血管生成，从而导致衰老过程中的代谢失调。作为对 THBS1 的反应，CD47 负向调节伤口愈合，抑制干细胞自我更新，并可能在膜运输和/或整合素依赖性信号转导中发挥作用。作为单体，CD47 与 THBS1、SIRPA、FAS/CD95、SIRPG、UBQLN1、UBQLN2 和潜在的纤维蛋白原相互作用，突出了其在细胞和分子途径中的复杂参与。

Immobilized Human SIRP alpha at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47 mFc-Avi with a linear range of 6-8 ng/mL.

Human

HEK293

C-mFc;C-Avi

Q08722-3 (Q19-P139)

961  [NCBI]

60-66 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.