CD47 蛋白, Mouse (HEK293, His)

CD47 Protein, an adhesive multifunctional protein, orchestrates cell-to-cell interactions, serving as a receptor for THBS1 and modulating integrin signaling via heterotrimeric G proteins. It plays a pivotal role in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, immunoregulation, and memory formation. CD47, a receptor for SIRPA, influences dendritic cell maturation, inhibits cytokine production, and mediates cell-cell adhesion. Additionally, it modulates pulmonary EDN1 signaling, supports blood pressure in response to THBS1-induced NO signaling, and contributes to memory formation. CD47's role in wound healing, stem cell self-renewal inhibition, and its interactions with THBS1, SIRPA, FAS/CD95, SIRPG, UBQLN1, UBQLN2, and possibly fibrinogen underscore its diverse impact on cellular processes. Existing as a monomer, CD47 exhibits intricate involvement in various cellular pathways. CD47 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD47 protein, expressed by HEK293, with C-6*His labeled tag. The total length of CD47 Protein, Mouse (HEK293, His) is 140 a.a., with molecular weight of 30-60 kDa.

CD47 蛋白是一种粘附蛋白，协调细胞间相互作用，并充当血小板反应蛋白 THBS1 的受体，同时通过异源三聚体 G 蛋白的激活调节整合素信号传导。这种多方面的蛋白质错综复杂地参与信号转导、心血管稳态、炎症、细胞凋亡、血管生成、细胞自我更新和免疫调节。CD47 在调节肺内皮素 EDN1 信号传导中发挥着关键作用，并作为升压剂支持血压，响应 THBS1 诱导的一氧化二氮 (NO) 信号传导。此外，它对海马体的记忆形成和突触可塑性有显着贡献。作为 SIRPA 的受体，CD47 可以阻止未成熟树突状细胞的成熟，抑制成熟树突状细胞产生细胞因子，并通过与 SIRPG 相互作用介导细胞间粘附。此外，它还能积极调节 T 细胞中 FAS 依赖性细胞凋亡并抑制血管生成，从而可能影响正常衰老过程中的代谢失调。CD47 在伤口愈合调节、干细胞自我更新抑制、膜运输的潜在参与、整合素依赖性信号转导以及防止红细胞过早消除中的作用强调了其对细胞过程的多种影响。CD47 作为单体存在，与 THBS1、SIRPA、FAS/CD95、SIRPG、UBQLN1、UBQLN2 以及可能的纤维蛋白原相互作用，强调其与多种细胞途径的复杂参与。

Mouse

HEK293

C-6*His

Q61735-2 (Q19-P158)

16423  [NCBI]

QLLFSNVNSIEFTSCNETVVIPCIVRNVEAQSTEEMFVKWKLNKSYIFIYDGNKNSTTTDQNFTSAKISVSDLINGIASLKMDKRDAMVGNYTCEVTELSREGKTVIELKNRTAFNTDQGSACSYEEEKGGCKLVSWFSP

30-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.