1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory Checkpoint Molecules Stem Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. Integrin Associated Protein/CD47
  5. CD47 蛋白, Mouse (Q61735-1, HEK293, His)

CD47 蛋白, Mouse (Q61735-1, HEK293, His)

目录号: HY-P72918
COA 产品使用指南

CD47 蛋白是一种粘附性多功能蛋白,可协调细胞间相互作用,充当 THBS1 受体,并通过异三聚体 G 蛋白调节整合素信号传导。它在信号转导、心血管稳态、炎症、细胞凋亡、血管生成、细胞自我更新、免疫调节和记忆形成中发挥着关键作用。CD47 蛋白, Mouse (Q61735-1, HEK293, His) 是重组的 CD47 蛋白,由 HEK293 表达,带有 C-His 标签。CD47 蛋白, Mouse (Q61735-1, HEK293, His) 全长 122 个氨基酸,分子量为 34-44 kDa。

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规格 价格 是否有货 数量
50 μg ¥3800 In-stock
100 μg   询价  

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描述

The CD47 protein is an adhesive multifunctional protein that coordinates cell-cell interactions, serves as a THBS1 receptor, and regulates integrin signaling through heterotrimeric G proteins. It plays a key role in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cell self-renewal, immune regulation, and memory formation. CD47 Protein, Mouse (Q61735-1, HEK293, His) is the recombinant mouse-derived CD47 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD47 Protein, Mouse (Q61735-1, HEK293, His) is 122 a.a., with molecular weight of 34-44 kDa.

研究背景

CD47 蛋白是一种粘附蛋白,协调细胞间相互作用,并充当血小板反应蛋白 THBS1 的受体,同时通过异源三聚体 G 蛋白的激活调节整合素信号传导。这种多方面的蛋白质错综复杂地参与信号转导、心血管稳态、炎症、细胞凋亡、血管生成、细胞自我更新和免疫调节。CD47 在调节肺内皮素 EDN1 信号传导中发挥着关键作用,并作为升压剂支持血压,响应 THBS1 诱导的一氧化二氮 (NO) 信号传导。此外,它对海马体的记忆形成和突触可塑性有显着贡献。作为 SIRPA 的受体,CD47 可以阻止未成熟树突状细胞的成熟,抑制成熟树突状细胞产生细胞因子,并通过与 SIRPG 相互作用介导细胞间粘附。此外,它还能积极调节 T 细胞中 FAS 依赖性细胞凋亡并抑制血管生成,从而可能影响正常衰老过程中的代谢失调。CD47 在伤口愈合调节、干细胞自我更新抑制、膜运输的潜在参与、整合素依赖性信号转导以及防止红细胞过早消除中的作用强调了其对细胞过程的多种影响。CD47 作为单体存在,与 THBS1、SIRPA、FAS/CD95、SIRPG、UBQLN1、UBQLN2 以及可能的纤维蛋白原相互作用,强调其与多种细胞途径的复杂参与。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized CD47 Protein, Mouse (HEK293, His) at 10μg/mL (100μL/well) can bind mouse SIRPA-Fc and the EC50 is 0.07-0.3 μg/mL.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q61735-1 (Q19-K140)

基因 ID

16423  [NCBI]

蛋白结构
N-term
CD47 (Q19-K140)
Accession # Q61735-1
His
C-term
同用名
Leukocyte Surface Antigen CD47; IAP; CD47; MER6
氨基酸序列

MWPLAAALLLGSCCCGSAQLLFSNVNSIEFTSCNETVVIPCIVRNVEAQSTEEMFVKWKLNKSYIFIYDGNKNSTTTDQNFTSAKISVSDLINGIASLKMDKRDAMVGNYTCEVTELSREGKTVIELKNRTVSWFSPNEK

分子量

34-44 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD47 Protein, Mouse (Q61735-1, HEK293, His)
目录号:
HY-P72918
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