CD58 蛋白, Human (HEK293, His)

CD58, a glycoprotein, is also known as lymphocyte-function antigen 3 (LFA-3). CD58 is a costimulatory receptor, and can interact with its natural ligand (CD2, primarily expressed on the surface of T/NK cells). The CD2-CD58 interaction can promote cell adhesion and recognition, and regulate antiviral responses, inflammatory responses in autoimmune diseases, immune rejection of transplantation, and immune evasion of tumor cells^[1]. CD58 Protein, Human (HEK293, His) is the recombinant human-derived CD58 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD58 Protein, Human (HEK293, His) is 187 a.a., with molecular weight of 30-60 kDa.

CD58，一种糖蛋白，也称为淋巴细胞功能抗原 3 (LFA-3)。CD58 是一种共刺激受体，分布在多种人体组织细胞上，例如T淋巴细胞、NK细胞、胸腺细胞和骨髓细胞亚群。CD58 可以与其天然配体 (CD2，主要表达于 T/NK 细胞表面) 相互作用。CD2-CD58 相互作用可以促进细胞粘附和识别，调节抗病毒反应、自身免疫性疾病的炎症反应、移植的免疫排斥和肿瘤细胞的免疫逃避^[1]。
CD58 有两种亚型：I 型跨膜形式和糖基磷脂酰肌醇 (GPI) 锚定形式。GPI 锚定的 CD58 形式在增强粘附方面更有效，但跨膜形式对于信号转导更为关键。此外，可溶形式的 CD58 (sCD58) 还存在于 in vitro 细胞上清液和 in vivo 局部组织中。sCD58 是一种免疫抑制因子，通过影响 CD2-CD58 相互作用参与 T/NK 细胞介导的免疫反应。sCD58 积累的改变可能导致肿瘤微环境中 T/NK 细胞的免疫抑制。因此，sCD58 可以作为一种新型免疫治疗靶点^[1]。

Measured in a cell proliferation assay using PHA-stimulated THP-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.4424 μg/mL,corresponding to a specific activity is 2.26×10³ units/mg.

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  Measured in a cell proliferation assay using PHA-stimulated THP‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.4424 μg/mL, corresponding to a specific activity is 2.26×10³ units/mg.

Human

HEK293

C-6*His

AAH05930 (F29-R215)

965  [NCBI]

FSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEYYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHR

30-60 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.