CD58 蛋白, Human (HEK293, Fc)

CD58 Protein, Human (HEK293, Fc) is the recombinant human-derived CD58 protein, expressed by HEK293, with C-hFc labeled tag.

CD58，一种糖蛋白，也称为淋巴细胞功能抗原 3 (LFA-3)。CD58 是一种共刺激受体，分布在多种人体组织细胞上，例如T淋巴细胞、NK细胞、胸腺细胞和骨髓细胞亚群。CD58 可以与其天然配体 (CD2，主要表达于 T/NK 细胞表面) 相互作用。CD2-CD58 相互作用可以促进细胞粘附和识别，调节抗病毒反应、自身免疫性疾病的炎症反应、移植的免疫排斥和肿瘤细胞的免疫逃避^[1]。
CD58 有两种亚型：I 型跨膜形式和糖基磷脂酰肌醇 (GPI) 锚定形式。GPI 锚定的 CD58 形式在增强粘附方面更有效，但跨膜形式对于信号转导更为关键。此外，可溶形式的 CD58 (sCD58) 还存在于 in vitro 细胞上清液和 in vivo 局部组织中。sCD58 是一种免疫抑制因子，通过影响 CD2-CD58 相互作用参与 T/NK 细胞介导的免疫反应。sCD58 积累的改变可能导致肿瘤微环境中 T/NK 细胞的免疫抑制。因此，sCD58 可以作为一种新型免疫治疗靶点^[1]。

1. Measured by its binding ability in a functional ELISA. Immobilized human CD2-His at 10 μg/mL (100 μl/well) can bind human CD58-Fc, The EC₅₀ of human CD58-Fc is 0.04-0.1 μg/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD2-His at 10 μg/mL (100 μl/well) can bind human CD58-Fc, The EC₅₀ of human CD58-Fc is 0.04-0.10 μg/mL.
3.Loaded Recombinant Human CD58/LFA-3 Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human CD2 Protein, His Tag with an affinity constant of 1.36 μM as determined in BLI assay.

Human

HEK293

C-hFc

Q9BRW0 (F29-R215)

965  [NCBI]

Extracellular Domain

Approximately 68 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.