1. 重组蛋白
  2. CD Antigens Complement System
  3. NK Cell CD Proteins Stem Cell CD Proteins Erythrocyte CD Proteins Complement Regulatory Proteins
  4. CD59
  5. CD59 蛋白, Human (HEK293, His)

CD59 蛋白, Human (HEK293, His)

目录号: HY-P72719
COA 产品使用指南

CD59 蛋白是补体膜攻击复合物 (MAC) 的有效抑制剂,可与组装的 C8 和/或 C9 补体结合,防止对渗透性孔形成至关重要的多个 C9 拷贝的掺入。其物种特异性抑制作用延伸至 T 细胞激活,与蛋白酪氨酸激酶复合以进行信号转导。CD59 蛋白, Human (HEK293, His) 是重组的 CD59 蛋白,由 HEK293 表达,带有 C-6*His 标签。CD59 蛋白, Human (HEK293, His) 全长 77 个氨基酸,分子量为 15-20 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1380 In-stock
50 μg ¥3950 询价
100 μg   询价  

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描述

The CD59 protein is a potent inhibitor of the complement membrane attack complex (MAC) and binds to assembled C8 and/or C9 complement, preventing the incorporation of multiple C9 copies that are critical for permeability pore formation. Its species-specific inhibitory effects extend to T cell activation, complexing with protein tyrosine kinases for signal transduction. CD59 Protein, Human (HEK293, His) is the recombinant human-derived CD59 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD59 Protein, Human (HEK293, His) is 77 a.a., with molecular weight of 15-20 kDa.

研究背景

CD59 蛋白是补体膜攻击复合物 (MAC) 作用的有效抑制剂。它通过与组装 MAC 的 C8 和/或 C9 补体结合来发挥作用,从而阻止渗透孔形成所需的 C9 多个拷贝的掺入。值得注意的是,该抑制剂表现出物种特异性。此外,CD59 与蛋白酪氨酸激酶复合参与信号转导的 T 细胞激活。值得注意的是,虽然尿液中的可溶性 CD59 保留了其特异性补体结合活性,但其抑制 MAC 在细胞膜上组装的能力显着降低。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P13987 (L26-N102)

基因 ID

966  [NCBI]

蛋白结构
N-term
CD59 (L26-N102)
Accession # P13987
6*His
C-term
同用名
CD59 glycoprotein; HRF-20; MAC-IP; MACIF; MIRL; CD59; MIC11; MIN1; MSK21
氨基酸序列

LQCYNCPNPTADCKTAVNCSSDFDACLITKAGLQVYNKCWKFEHCNFNDVTTRLRENELTYYCCKKDLCNFNEQLEN

分子量

15-20 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD59 Protein, Human (HEK293, His)
目录号:
HY-P72719
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