1. 重组蛋白
  2. CD Antigens
  3. Dendritic Cell CD Proteins Cell Adhesion-related CD Proteins
  4. CD6
  5. CD6 蛋白, Rat (HEK293, His)

CD6 蛋白, Rat (HEK293, His)

目录号: HY-P72924
COA 产品使用指南

CD6 是一种蛋白质,缺乏传播特征注释所需的重要保守残基。CD6 蛋白, Rat (HEK293, His) 是重组的 CD6 蛋白,由 HEK293 表达,带有 C-His 标签。CD6 蛋白, Rat (HEK293, His) 全长 379 个氨基酸,分子量约为 ~60-90 kDa。

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规格 价格 是否有货 数量
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5 μg ¥350 In-stock
10 μg ¥590 In-stock
50 μg ¥1470 In-stock
100 μg ¥2200 In-stock
500 μg ¥5500 In-stock
1 mg ¥8800 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CD6, a protein, lacks essential conserved residue(s) necessary for propagating feature annotation. CD6 Protein, Rat (HEK293, His) is the recombinant rat-derived CD6 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD6 Protein, Rat (HEK293, His) is 379 a.a., with molecular weight of ~60-90 kDa.

研究背景

CD6 缺乏特征注释传播所必需的保守残基。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to rat CD6-His coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/mL PHA, 72.012% cells adhered specifically after 60 minutes at 37°C.

种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

Q812A4 (L18-N396)

基因 ID

25752  [NCBI]

蛋白结构
N-term
CD6 (L18-N396)
Accession # Q812A4
His
C-term
同用名
T-cell differentiation antigen CD6; T12; TP120; CD6
氨基酸序列

LPPPAPSDQHRNGSIPNMSSDPEEQLGIRLVNGSSRCSGFVQVLLESWEPVCAGHWNRAATEAACNALGCGDLGNFTHLAPPTSERPPGATSRNTSSSRNTTWAGAPTVRCHGANWQLCKVEDYECSSDRRLVWVTCAENQAVRLVDGGSRCAGRVEMLQHGEWGTVCDDTWDLDDANVVCKQLKCGWAVKALAGLHFTPGQGPIHRDQVNCSGTEAYLWDCPGRPGDQYCGHKEDAGVVCSEHQSWRLTGGVDSCEGQVEVYFRGVWSTVCDSEWYPSEAKVLCQSLGCGSEVDRPKGLPHSLAGRMYYSCKGEELTLSTCSWRFNNSNLCSQSLAARVVCSGSQRHPNLSTSEVPSRVPVTIESSVPVSVKDKDSQN

分子量

Approximately 60-90 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD6 Protein, Rat (HEK293, His)
目录号:
HY-P72924
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