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  2. Immune Checkpoint Proteins CAR-T related Proteins CD Antigens
  3. Stimulatory Immune Checkpoint Molecules NK Cell CD Proteins Macrophage CD Proteins
  4. CD70
  5. CD70 蛋白, Cynomolgus (HEK293, His)

CD70 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P77620
COA 产品使用指南

CD70 是活化 T 和 B 淋巴细胞上的表面抗原,是 CD27 的配体,参与 CD70-CD27 通路和 T 细胞免疫的产生和维持。CD70 具有抗病毒活性,并调节肿瘤细胞和调节 T 细胞的活力。而来源食蟹猴的 CD70 蛋白含有一个 TNF_2 结构域 (57-189 a.a) 和跨膜螺旋,属于肿瘤坏死因子 (TNF) 家族。重组食蟹猴 CD70 蛋白 (HEK293, His) 全长 156 个氨基酸 (Q39-P194),在 HEK293 细胞中表达,并带有 N 端 His 标签。

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规格 价格 是否有货 数量
20 μg ¥1860 In-stock
50 μg ¥4100 In-stock
100 μg   询价  

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描述

CD70 is the ligand of CD27 in activated T and B lymphocytes, is an important cytokine in CD70-CD27 pathway involving with the generation and maintenance of T cell immunity[1]. CD70 is a surface antigen, also shows antiviral activity and regulates viability of tumor cells and regulatory T cells[2][3]. As for CD70 protein in cynomolgus contains TNF_2 domain (57-189 a.a) and transmembrane helix, belonging to the tumor necrosis factor (TNF) family. CD70 Protein, Cynomolgus (HEK293, His) is 156 amino acids in length (Q39-P194) and is expressed in the HEK293 cells with a N terminal His-tag.

研究背景

CD70 (CD27 Ligand)属于肿瘤坏死因子(TNF)家族,是TNFRSF27/CD27[1]的配体。
CD70和CD27是II型同源三聚体和I型同源二聚体跨膜糖蛋白,分别在激活的 T 淋巴细胞和静息的 T 淋巴细胞和 B 淋巴细胞上表达[3][4]CD70 作为一系列弥漫性大 B 细胞淋巴瘤中最常见的突变基因之一,尤其在关键的 Epstein-Barr 病毒 (EBV) 特异性 T 细胞免疫中发挥作用,更广泛地用于 B 细胞的免疫监视。CD70 通过恢复由 CD70 缺陷的 EBV 感染 B 细胞刺激的 EBV 特异性 T 淋巴细胞的扩增来抑制 EBV 感染[3]
CD70 参与先天性和适应性免疫的激活,在成熟树突状细胞中表达,并在 CD40 或 Toll 样受体的触发下上调[2]
CD70 诱导共刺激 T 细胞增殖,增强溶细胞 T 细胞的生成细胞,并有助于 T 细胞激活[4]
据报道,CD70 在调节 B 细胞激活、自然杀伤细胞的细胞毒功能和免疫球蛋白合成中发挥作用[5]。使用 CAR-T 细胞靶向 CD70 阳性肿瘤可诱导有效的抗肿瘤反应[6]

生物活性

Immobilized Cynomolgus CD27 Ligand at 2 μg/mL (100μL/Well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27 with the EC50 of 60.8 ng/mL determined by ELISA.

种属

Cynomolgus

表达系统

HEK293

标签

N-His

蛋白编号

G7PYU6 (Q39-P194)

基因 ID

/

蛋白结构
N-term
His
CD70 (Q39-P194)
Accession # G7PYU6
C-term
中文名
重组食蟹猴 CD70 蛋白 (HEK293, His)
同用名
CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;  Ki-24 antigen; TNFSF7G
分子量

60-90 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD70 Protein, Cynomolgus (HEK293, His)
目录号:
HY-P77620
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