CD73/5'-Nucleotidase 蛋白, Human (521a.a, HEK293, His)

CD73/5'-Nucleotidase Protein hydrolyzes nucleotide monophosphates, favoring AMP as the primary substrate and showing a preference for ribonucleotide monophosphates. It releases inorganic phosphate and the corresponding nucleoside. CD73 also acts on other substrates, including IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN. CD73/5'-Nucleotidase Protein, Human (521a.a, HEK293, His) is the recombinant human-derived CD73/5'-Nucleotidase protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD73/5'-Nucleotidase Protein, Human (521a.a, HEK293, His) is 521 a.a., with molecular weight of ~62.0 kDa.

CD73/5'-核苷酸酶蛋白通过催化单磷酸核苷酸的水解、释放无机磷酸盐和相应的核苷，在细胞过程中发挥着至关重要的作用。值得注意的是，AMP 是该酶的首选底物，强调了它在腺苷酸核苷酸转化中的重要性。CD73/5'-核苷酸酶表现出对核糖核苷酸单磷酸的偏好超过其脱氧核糖对应物，显示其在底物识别中的选择性。除AMP外，其他底物还包括IMP、UMP、GMP、CMP、dAMP、dCMP、dTMP、NAD和NMN，进一步说明CD73/5'-核苷酸酶在核苷酸代谢中的多功能性及其调节核苷酸平衡的作用细胞内的池。

1. 2 μg/mL (100 μL/well) of immobilized recombinant Human 5'-nucleotidase/CD73-His can bind Anti-Human CD73 mAb with an ED₅₀ value of 2.78 ng/mL.
2. Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is about 3×10⁵ pmol/min/μg.

Human

HEK293

C-6*His

P21589 (W27-K547)

4907  [NCBI]

WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

Approximately 62.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl₂, 20% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.