CD9 Protein-VLP, Human (HEK293)

CD9 protein is an integral membrane protein that plays a critical regulatory role in processes such as sperm-egg fusion, platelet activation, and cell adhesion. CD9 is critical for sperm-egg fusion, coordinating multi-protein complexes on the oocyte surface. CD9 Protein-VLP, Human (HEK293) is the recombinant human-derived CD9 protein, expressed by HEK293, with tag free.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.

CD9 是一种完整的膜蛋白，在调节各种生物过程中发挥着关键作用，包括精卵融合、血小板活化和聚集以及细胞粘附。CD9 位于卵母细胞的细胞表面，对于精卵融合至关重要，可能通过协调融合过程所需的多蛋白复合物和膜形态来实现。在成肌细胞中，CD9 与 CD81 和 PTGFRN 结合，抑制肌肉再生过程中的肌管融合。在巨噬细胞内，CD9 与 CD81 和 beta-1/beta-2 整合素结合，防止巨噬细胞融合成专门摄取补体调理大颗粒的多核巨细胞。CD9 还阻碍单核细胞祖细胞融合成负责骨吸收的破骨细胞。除了在融合事件中的作用外，CD9 还充当 PSG17 的受体，并与血小板活化、聚集、节旁连接形成、细胞粘附、细胞运动和肿瘤转移有关。CD9 形成二硫键连接的同源二聚体和高级同源寡聚体，以及与其他四跨膜蛋白家族成员的异源寡聚体。它与整合素 ITGAV:ITGB3 和 ITGA6:ITGB1 相互作用，并且是单核细胞/巨噬细胞膜中整合素-四跨膜蛋白复合物的一部分。CD9 与 CD63、PTGFRN、IGSF8、CR2/CD21 和 PDPN 形成复合物，在各种细胞功能和相互作用中发挥着至关重要的作用。

Human

HEK293

Tag Free

P21926 (P2-V228)

928

Full Length

溶液.

Supplied as 0.2 μm filtered solution of PBS, Arginine, pH7.4 with trehalose as protectant.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.