CD94 蛋白, Human (HEK293, His)

CD94 protein is a key immune receptor for self-non-self discrimination. It forms a complex with KLRC1 or KLRC2 on lymphocyte subsets and recognizes HLA-E loaded with self-peptides. It allows cytotoxic cells to monitor MHC class I expression and promote self-tolerance. CD94 Protein, Human (HEK293, His) is the recombinant human-derived CD94 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of CD94 Protein, Human (HEK293, His) is 146 a.a., with molecular weight of 23-28 kDa.

CD94 蛋白是一种对自我-非自我辨别至关重要的免疫受体，与细胞毒性和调节性淋巴细胞亚群上的 KLRC1 或 KLRC2 形成复合物，识别非经典主要组织相容性 (MHC) Ib 类分子 HLA-E，该分子负载有源自自体的肽经典 MHC Ia 类和其他非经典 MHC Ib 类分子的信号序列。这种相互作用使细胞毒性细胞能够监测健康细胞中 MHC I 类的表达，从而促进自我耐受。KLRD1-KLRC1 复合物主要作为配体结合亚基，不具有信号传递能力，可作为免疫抑制受体，其中 CD94 对自然杀伤 (NK) 细胞发挥关键的抑制作用。CD94 主要抵消记忆/效应 CD8 阳性 T 细胞子集上的 T 细胞受体信号传导，以预防自身免疫。在上皮内 CD8 阳性 γ-δ 调节性 T 细胞上，CD94 触发 TGFB1 分泌，限制上皮内 CD8 阳性 α-β T 细胞的细胞毒性编程，并将无害抗原与致病抗原区分开来。在富含 HLA-E 的肿瘤微环境中，CD94 充当免疫抑制检查点，可能导致 NK 细胞和肿瘤特异性 T 细胞效应功能逐渐丧失，这种状态称为细胞衰竭。HLA-E 肽结合后，CD94 通过 KLRC1 免疫受体酪氨酸抑制基序 (ITIM) 传递细胞内信号，将 INPP5D/SHIP-1 和 INPPL1/SHIP-2 酪氨酸磷酸酶募集到 ITIM，最终通过去磷酸化近端来对抗来自激活受体的信号信号分子。

Human

HEK293

N-6*His

Q13241 (S34-I179)

3824  [NCBI]

SFTKLSIEPAFTPGPNIELQKDSDCCSCQEKWVGYRCNCYFISSEQKTWNESRHLCASQKSSLLQLQNTDELDFMSSSQQFYWIGLSYSEEHTAWLWENGSALSQYLFPSFETFNTKNCIAYNPNGNALDESCEDKNRYICKQQLI

23-28 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.