1. 重组蛋白
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  3. CDC37 蛋白, Mouse (sf9)

CDC37 蛋白是一种共伴侣,可与激酶结合并促进激酶与 Hsp90 复合物的相互作用,从而稳定和激活它们。它抑制 HSP90AA1 的 ATP 酶活性。CDC37 蛋白, Mouse (sf9) 是重组的 CDC37 蛋白,由 Sf9 insect cells 表达,不带标签。

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描述

The CDC37 protein is a co-chaperone that binds to kinases and promotes their interaction with Hsp90 complexes, thereby stabilizing and activating them. It inhibits the ATPase activity of HSP90AA1. CDC37 Protein, Mouse (sf9) is the recombinant mouse-derived CDC37 protein, expressed by Sf9 insect cells, with tag free.

研究背景

CDC37 蛋白是一种共伴侣,可与各种激酶结合并促进它们与 Hsp90 复合物的相互作用,从而稳定和激活这些激酶。它抑制 HSP90AA1 的 ATP 酶活性。它可能形成由伴侣 HSP90 和 HSP70、辅助伴侣 STIP1/HOP、CDC37、PPP5C、PTGES3/p23、TSC1 和客户蛋白 TSC2 组成的复合物。该复合体还可能包括 CDK4、AKT、RAF1 和 NR3C1,但不包含 STIP1/HOP 和 PTGES3/p23。CDC37 与 Hsp90/HSP90AB1 和 CDK6 形成单独的复合物。它与多种蛋白质相互作用,包括 HSP90AA1、AR、CDK4、CDK6、EIF2AK1、RB1、KSR1、FLCN、FNIP1 和 FNIP2。

种属

Mouse

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q61081 (N-G&P, M1-A379)

基因 ID
蛋白长度

Full Length

中文名
CDC37 蛋白
同用名
Hsp90 co-chaperone Cdc37; CDC37; CDC37A
预测分子量
44.7 kDa
分子量

Approximately 46 kDa,based on SDS-PAGE under reducing conditions.

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CDC37 Protein, Mouse (sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CDC37 Protein, Mouse (sf9)
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