CDK16-CCNY 蛋白, Human (sf9, GST, Flag)

PCTAIRE1 protein is a multifunctional kinase that plays a key role in vesicle-mediated transport and regulation of GH1 release, emphasizing neuroendocrine function. Its involvement includes NSF phosphorylation, influencing vesicle dynamics, and important contributions to spermatogenesis and reproductive processes. CDK16-CCNY Protein, Human (sf9, GST, Flag) is the recombinant human-derived CDK16-CCNY, expressed by Sf9 insect cells, with N-GST, N-Flag labeled tag.

PCTAIRE1 蛋白是一种多功能激酶，在多种细胞过程中发挥着关键作用，特别是在囊泡介导的运输和胞吐作用中。它的参与延伸到脑神经元 GH1 释放的调节，强调了它在神经内分泌功能中的重要性。PCTAIRE1 的作用范围包括 NSF 的磷酸化、调节 NSF 寡聚化，从而影响囊泡动力学的关键方面。值得注意的是，它是精子发生的重要参与者，有助于正常的生殖过程。此外，PCTAIRE1 在神经元分化和树突发育的复杂调节中发挥着关键作用，强调了它对神经元形态的影响。除了其神经元功能之外，PCTAIRE1 还参与响应血糖水平变化而调节胰岛素分泌，从而暗示其在代谢稳态中的作用。该激酶在体外磷酸化 CCNY 'Ser-336' 的能力进一步凸显了其多样化的分子相互作用，并强调了其在细胞信号传导中的多方面作用。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The Km of CDK16-CCNY for its substrate is 20 μM.

Human

Sf9 insect cells

N-GST;N-Flag

Q00536-1 (M1-F496)&Q8ND76-1 (M1-S341)

5127&219771

82.3 kDa & 41.3 kDa

Greater than 80 % as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 5% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.