CENPE 蛋白, Human

CENPE Protein, Human is the recombinant human-derived CENPE, expressed by E. coli , with tag Free labeled tag.

CENPE 是一种微管正端导向的动粒马达蛋白，在染色体列队 (chromosome congression)、微管-动粒连接 (microtubule-kinetochore conjugation) 和纺锤体组装检查点激活 (spindle assembly checkpoint activation) 中起关键作用。它通过介导极区染色体沿纺锤体微管向赤道板的侧向滑动，并协助建立和维持动粒与纺锤体微管之间的连接，从而驱动染色体列队 (形成中期板)。去酪氨酸化的微管轨道更有利于近极染色体向赤道板的运输。CENPE 作为动态微管末端的双向追踪器，在染色体完成列队后仍持续增强动粒与动态微管末端的连接。在 NDC80 缺失细胞中会抑制染色体列队，且仅当微管稳定时才对列队起促进作用。它对形成动粒-微管稳定连接至关重要，该过程还需要 CENPE 依赖性定位其他蛋白 (如 BUB1B、MAD1 和 MAD2) 至动粒。通过与 BUB1B 相互作用激活其激酶活性，进而激活纺锤体组装检查点 (SAC)，在防止单染色体丢失导致的非整倍性中发挥作用 (by similar)。

Human

E. coli

Tag Free

Q02224 (M1-S339)

1062

Partial

Approximately 38.2 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.