Cerebellin-2 蛋白, Human (CHO, HA)

The protein Cerebellin-2 organizes synapses in specific brain neurons, which is critical for long-term maintenance. It forms homohexamers composed of disulfide-bonded homotrimers connected through the C1q domain. Cerebellin-2 Protein, Human (CHO, HA) is the recombinant human-derived Cerebellin-2 protein, expressed by CHO , with N-HA labeled tag.

Cerebellin-2 蛋白作为大脑特定神经元亚群中的突触组织者，在兴奋性突触的长期维持（但不是建立）中发挥着重要作用。它形成由二硫键结合的同源三聚体连接的同源六聚体，通过球状 C1q 结构域组装，三聚体通过 N 端半胱氨酸残基缔合，形成二硫键连接的六聚体。Cerebellin-2 还可以在分泌前与 CBLN1 和 CBLN3 形成同寡聚体或异寡聚体。一旦分泌，它就不再与其他 CBLN 家族成员相互作用。Cerebellin-2 与 GRID2 相互作用，并在较小程度上与 GRID1 相互作用。此外，它还与通过替代启动子使用生成的 NRXN1 和 NRXN2 长亚型和短亚型相互作用。该蛋白与 NRXN3 短异构体表现出弱相互作用，与 NRXN3 长异构体没有相互作用。

Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ for this effect is 1.222 μg/mL, corresponding to a specific activity is 818.331 units/mg.

Human

CHO

N-HA

Q8IUK8 (R50-L224)

147381  [NCBI]

Full Length of Mature Protein

Approximately 30 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.