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  3. Cerebellin-2 蛋白, Human (CHO, HA)

Cerebellin-2 蛋白, Human (CHO, HA)

目录号: HY-P79253
COA 产品使用指南 技术支持

蛋白质 Cerebellin-2 在特定的大脑神经元中组织突触,这对于长期维持至关重要。它形成由通过 C1q 结构域连接的二硫键同源三聚体组成的同源六聚体。Cerebellin-2 蛋白, Human (CHO, HA) 是重组的 小脑蛋白 2 蛋白,由 CHO 表达,带有 N-HA 标签。

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5 μg ¥900 In-stock
10 μg ¥1350 In-stock
50 μg ¥3600 In-stock
100 μg ¥5760 In-stock
500 μg ¥14400 In-stock
1 mg ¥21600 In-stock
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  • 生物活性

  • 技术参数

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描述

The protein Cerebellin-2 organizes synapses in specific brain neurons, which is critical for long-term maintenance. It forms homohexamers composed of disulfide-bonded homotrimers connected through the C1q domain. Cerebellin-2 Protein, Human (CHO, HA) is the recombinant human-derived Cerebellin-2 protein, expressed by CHO , with N-HA labeled tag.

研究背景

Cerebellin-2 蛋白作为大脑特定神经元亚群中的突触组织者,在兴奋性突触的长期维持(但不是建立)中发挥着重要作用。它形成由二硫键结合的同源三聚体连接的同源六聚体,通过球状 C1q 结构域组装,三聚体通过 N 端半胱氨酸残基缔合,形成二硫键连接的六聚体。Cerebellin-2 还可以在分泌前与 CBLN1 和 CBLN3 形成同寡聚体或异寡聚体。一旦分泌,它就不再与其他 CBLN 家族成员相互作用。Cerebellin-2 与 GRID2 相互作用,并在较小程度上与 GRID1 相互作用。此外,它还与通过替代启动子使用生成的 NRXN1 和 NRXN2 长亚型和短亚型相互作用。该蛋白与 NRXN3 短异构体表现出弱相互作用,与 NRXN3 长异构体没有相互作用。

生物活性

Measured in a cell proliferation assay using SH-SY5Y cells. The ED50 for this effect is 1.222 μg/mL, corresponding to a specific activity is 818.331 units/mg.

种属

Human

表达系统

CHO

标签

N-HA

蛋白编号

Q8IUK8 (R50-L224)

基因 ID
蛋白结构
N-term
HA
Cerebellin-2 (R50-L224)
Accession # Q8IUK8
C-term
蛋白长度

Full Length of Mature Protein

中文名
Cerebellin-2 蛋白
同用名
Cerebellin-2; CBLN2
预测分子量
20.2 kDa
分子量

Approximately 30 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

糖基化修饰
Yes
纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 200 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Cerebellin-2 Protein, Human (CHO, HA) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Cerebellin-2 Protein, Human (CHO, HA)
目录号:
HY-P79253
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