CES2/carboxylesterase 2 蛋白, Human (HEK293,C-His,Solution)

CES2 Protein plays a crucial role in detoxifying xenobiotics and activating ester and amide prodrugs. It exhibits high catalytic efficiency, particularly in the hydrolysis of substances like cocaine, 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. Additionally, CES2 participates in the hydrolysis of aspirin, substrates with large alcohol groups and small acyl groups, as well as endogenous lipids such as triacylglycerol. The protein is involved in the conversion of monoacylglycerides to free fatty acids and glycerol and contributes to the hydrolysis of 2-arachidonoylglycerol and prostaglandins. CES2/carboxylesterase 2 Protein, Human (HEK293,C-His,Solution) is the recombinant human-derived CES2/carboxylesterase 2 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of CES2/carboxylesterase 2 Protein, Human (HEK293,C-His,Solution) is 533 a.a., with molecular weight of ~50-70 kDa.

CES2/羧酸酯酶 2 蛋白在外源物质的解毒以及酯和酰胺前药的激活中发挥着至关重要的作用。它在多种化合物的水解中表现出高催化效率，包括可卡因、4-甲基伞形基乙酸酯、海洛因和 6-单乙酰吗啡。此外，CES2 还可以水解阿司匹林、具有大醇基和小酰基的底物，以及内源性脂质，例如三酰甘油。值得注意的是，它参与单酰基甘油酯向游离脂肪酸和甘油的转化。此外，CES2 还表现出 2-花生四烯酰甘油和前列腺素水解活性，突出了其酶功能的多功能性，对各种生物过程和药物代谢具有潜在影响。

Measured by its ability to hydrolyze p-nitrophenylacetate.Which can be measured by absorbance at 410 nm. The specific activity is 42718.666 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-10*His

O00748-1 (Q27-L559)

8824

QDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL

Approximately 50-70 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM NaAc, 150 mM NaCl, 10% Glycerol, pH 5.5.

<1 EU/μg, determined by LAL method

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.