2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. CES3/CES1D 蛋白, Mouse (HEK293, His)

CES3/CES1D 蛋白, Mouse (HEK293, His)

目录号: HY-P70088
产品使用指南

CES3/CES1D 蛋白是白色脂肪组织中的主要脂肪酶，在外源物质和天然底物代谢中发挥着至关重要的作用。它水解三酰基甘油和单酰基甘油，显示出对后者的偏好，敏感性随着酰基链长度的减小而增加。此外，CES3/CES1D 还可催化脂肪酸乙酯的合成并水解视黄酯。CES3/CES1D 蛋白, Mouse (HEK293, His) 是重组的 CES3/CES1D 蛋白，由 HEK293 表达，带有 C-6*His 标签。CES3/CES1D 蛋白, Mouse (HEK293, His) 全长 543 个氨基酸，分子量为 58-70 kDa。

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规格 是否有货
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100 μg   询价  

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描述

CES3/CES1D Protein, a major lipase in white adipose tissue, plays a crucial role in xenobiotic and natural substrate metabolism. It hydrolyzes triacylglycerols and monoacylglycerols, showing a preference for the latter, with susceptibility increasing as the acyl chain length decreases. Additionally, CES3/CES1D catalyzes the synthesis of fatty acid ethyl esters and hydrolyzes retinyl esters. CES3/CES1D Protein, Mouse (HEK293, His) is the recombinant mouse-derived CES3/CES1D protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CES3/CES1D Protein, Mouse (HEK293, His) is 543 a.a., with molecular weight of 58-70 kDa.
研究背景

CES3/CES1D 蛋白是一种主要脂肪酶，主要存在于白色脂肪组织中，在外源物质和天然底物代谢中发挥着至关重要的作用。该酶参与三酰基甘油和单酰基甘油的水解，表现出对后者的偏好。其底物敏感性随着脂肪酸部分酰基链长度的减少而增加。CES3/CES1D还催化脂肪酸乙酯的合成并参与视黄酯的水解。
种属

Mouse
表达系统

HEK293
标签

C-6*His
蛋白编号

Q8VCT4 (Y19-E561)
基因 ID

104158  [NCBI]
蛋白结构
N-term
CES3 (Y19-E561)
Accession # Q8VCT4
6*His
C-term
同用名
rMuCarboxylesterase 1D/CES3, His; CES3; carboxylesterase 3; carboxylesterase 3 (brain); EC 3.1.1; EC 3.1.1.1; ES31FLJ21736; Esterase 31; Liver carboxylesterase 31 homolog
氨基酸序列

YPSSPPVVNTVKGKVLGKYVNLEGFTQPVAVFLGVPFAKPPLGSLRFAPPQPAEPWSFVKNTTSYPPMCSQDAVGGQVLSELFTNRKENIPLQFSEDCLYLNIYTPADLTKNSRLPVMVWIHGGGLVVGGASTYDGLALSAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIANFGGNPGSVTIFGESAGGFSVSVLVLSPLAKNLFHRAISESGVSLTAALITTDVKPIAGLVATLSGCKTTTSAVMVHCLRQKTEDELLETSLKLNLFKLDLLGNPKESYPFLPTVIDGVVLPKAPEEILAEKSFSTVPYIVGINKQEFGWIIPTLMGYPLAEGKLDQKTANSLLWKSYPTLKISENMIPVVAEKYLGGTDDLTKKKDLFQDLMADVVFGVPSVIVSRSHRDAGASTYMYEFEYRPSFVSAMRPKAVIGDHGDEIFSVFGSPFLKDGASEEETNLSKMVMKFWANFARNGNPNGGGLPHWPEYDQKEGYLKIGASTQAAQRLKDKEVSFWAELRAKESAQRPSHRE

分子量

58-70 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CES3/CES1D Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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