1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. CHAC1 蛋白, Human

CHAC1 蛋白, Human

目录号: HY-P701945
产品使用指南

CHAC1 蛋白裂解谷胱甘肽,通过 ATF4-ATF3-DDIT3/CHOP 级联诱导细胞凋亡。它作为 Notch 信号通路的负调节因子,通过抑制 Notch 裂解来促进神经发生。CHAC1 蛋白, Human 是重组的 CHAC1 蛋白,由 E. coli 表达,不带标签。CHAC1 蛋白, Human 全长 221 个氨基酸。

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描述

The CHAC1 protein cleaves glutathione, inducing apoptosis via the ATF4-ATF3-DDIT3/CHOP cascade. It acts as a negative regulator of the Notch signaling pathway, promoting neurogenesis by inhibiting Notch cleavage. CHAC1 Protein, Human is the recombinant human-derived CHAC1 protein, expressed by E. coli , with tag free. The total length of CHAC1 Protein, Human is 221 a.a., .

研究背景

CHAC1 是细胞过程中的关键参与者,可催化谷胱甘肽裂解为 5-氧代-L-脯氨酸和 Cys-Gly 二肽,与其他 γ-谷氨酰肽相比,对谷胱甘肽表现出特异性。其对谷胱甘肽的酶促作用至关重要,因为谷胱甘肽的消耗是细胞凋亡启动和执行的重要因素。CHAC1 进一步充当未折叠蛋白反应途径中的促凋亡成分,介导 ATF4-ATF3-DDIT3/CHOP 级联的作用。此外,CHAC1 作为 Notch 信号通路的负调节因子,通过弗林蛋白酶抑制 Notch 裂解,从而影响胚胎神经发生。这种抑制使Notch维持在不成熟、不活跃的状态,从而促进胚胎中的神经发生。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q9BUX1 (K2-V222)

基因 ID

79094

蛋白结构
N-term
CHAC1 (K2-V222)
Accession # Q9BUX1
C-term
同用名
CHAC1; Glutathione-specific gamma-glutamylcyclotransferase 1; Gamma-GCG 1; Blocks Notch protein; Botch; Cation transport regulator-like protein 1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CHAC1 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CHAC1 Protein, Human
目录号:
HY-P701945
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