CHI3L1 蛋白, Human (HEK293, His)

The CHI3L1 protein is a binding lectin that lacks chitinase activity. CHI3L1 contributes to tissue remodeling, cellular adaptation to environmental changes, and T helper type 2 inflammatory responses. CHI3L1 also controls hyperoxia-induced injury, inflammation, and epithelial cell apoptosis. CHI3L1 Protein, Human (HEK293, His) is the recombinant human-derived CHI3L1 protein, expressed by HEK293 , with C-6*His labeled tag.

几丁质酶 3 样蛋白 1 (CHI3L1) 是一种碳水化合物结合凝集素，对几丁质具有特异性亲和力，尽管它缺乏几丁质酶活性。它的主要作用超出了几丁质降解，因为它涉及组织重塑和细胞对环境变化的反应。CHI3L1 在 2 型辅助性 T 细胞 (Th2) 炎症反应和 IL-13 诱导的炎症反应中发挥着至关重要的作用，影响过敏原致敏、炎症细胞凋亡、树突状细胞积累和 M2 巨噬细胞的分化。此外，它还促进致病性肠道细菌侵入结肠粘膜和淋巴器官。CHI3L1 参与 AKT1 信号通路的激活，导致结肠上皮细胞产生 IL8。此外，它还调节肺部的抗菌反应，有助于杀死巨噬细胞细菌、控制细菌传播并增强宿主耐受性。在肺组织中，CHI3L1 还在调节高氧诱导的损伤、炎症和上皮细胞凋亡中发挥作用。在结构上，CHI3L1 作为单体发挥作用。

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 0.6-3.0 μg/mL.

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  Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 2.603 μg/mL, corresponding to a specific activity is 384.1721 units/mg.

Human

HEK293

C-6*His

P36222 (Y22-T383)

1116  [NCBI]

Full Length of Mature Protein

YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT

Approximately 41-46 kDa band in SDS-PAGE under reducing conditions due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 2 mM EDTA, pH 7.4 or 20 mM Tris-HCl, 300 mM NaCl, 2 mM EDTA, pH8.5 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.