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  3. CHI3L1 蛋白, Human (HEK293, His)

CHI3L1 蛋白, Human (HEK293, His)

目录号: HY-P70030
COA 产品使用指南 技术支持

CHI3L1 蛋白是一种缺乏几丁质酶活性的结合凝集素。CHI3L1 有助于组织重塑、细胞对环境变化的适应以及 T 辅助细胞 2 型炎症反应。CHI3L1 还控制高氧诱导的损伤、炎症和上皮细胞凋亡。CHI3L1 蛋白, Human (HEK293, His) 是重组的 CHI3L1 蛋白,由 HEK293 表达,带有 C-6*His 标签。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥350 In-stock
10 μg ¥900 In-stock
50 μg ¥2700 In-stock
100 μg ¥4500 询价
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The CHI3L1 protein is a binding lectin that lacks chitinase activity. CHI3L1 contributes to tissue remodeling, cellular adaptation to environmental changes, and T helper type 2 inflammatory responses. CHI3L1 also controls hyperoxia-induced injury, inflammation, and epithelial cell apoptosis. CHI3L1 Protein, Human (HEK293, His) is the recombinant human-derived CHI3L1 protein, expressed by HEK293 , with C-6*His labeled tag.

研究背景

几丁质酶 3 样蛋白 1 (CHI3L1) 是一种碳水化合物结合凝集素,对几丁质具有特异性亲和力,尽管它缺乏几丁质酶活性。它的主要作用超出了几丁质降解,因为它涉及组织重塑和细胞对环境变化的反应。CHI3L1 在 2 型辅助性 T 细胞 (Th2) 炎症反应和 IL-13 诱导的炎症反应中发挥着至关重要的作用,影响过敏原致敏、炎症细胞凋亡、树突状细胞积累和 M2 巨噬细胞的分化。此外,它还促进致病性肠道细菌侵入结肠粘膜和淋巴器官。CHI3L1 参与 AKT1 信号通路的激活,导致结肠上皮细胞产生 IL8。此外,它还调节肺部的抗菌反应,有助于杀死巨噬细胞细菌、控制细菌传播并增强宿主耐受性。在肺组织中,CHI3L1 还在调节高氧诱导的损伤、炎症和上皮细胞凋亡中发挥作用。在结构上,CHI3L1 作为单体发挥作用。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 0.6-3.0 μg/mL.

  • Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 2.603 μg/mL, corresponding to a specific activity is 384.1721 units/mg.
种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P36222 (Y22-T383)

基因 ID
蛋白结构
N-term
CHI3L1 (Y22-T383)
Accession # P36222
6*His
C-term
蛋白长度

Full Length of Mature Protein

中文名
CHI3L1 蛋白
同用名
rHuChitinase-3-like protein 1/CHI3L1, His; Chitinase-3-Like protein 1; 39 kDa Synovial Protein; Cartilage Glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40; CHI3L1
氨基酸序列

YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT

预测分子量
41.3 kDa
分子量

Approximately 41-46 kDa band in SDS-PAGE under reducing conditions due to the glycosylation.

糖基化修饰
Yes
纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 2 mM EDTA, pH 7.4 or 20 mM Tris-HCl, 300 mM NaCl, 2 mM EDTA, pH8.5 or PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CHI3L1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CHI3L1 Protein, Human (HEK293, His)
目录号:
HY-P70030
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