CIB2 蛋白, Human (His)

CIB2 protein: Calcium and integrin-binding protein involved in intracellular calcium regulation, auditory hair cell mechanotransduction, and maintenance of stereocilia bundle morphology. CIB2 Protein, Human (His) is the recombinant human-derived CIB2 protein, expressed by E. coli , with N-His labeled tag. The total length of CIB2 Protein, Human (His) is 187 a.a., with molecular weight of ~26 kDa.

CIB2 蛋白是一种钙和整合素结合蛋白，在细胞内钙稳态中发挥着至关重要的作用，并作为毛细胞中感觉机电转导 (MET) 通道的辅助亚基发挥作用。它对于听觉毛细胞中的机电传导电流的重要性强调了它在听力中的重要性。CIB2 通过影响跨膜通道样蛋白 TMC1 和 TMC2 的分布以及调节毛细胞中 MET 通道的活性来调节毛细胞机械转导的功能。而且，它对于维持听毛细胞静纤毛束的形态和功能，保证耳蜗内毛细胞的存活是不可缺少的。此外，CIB2 在感光细胞的维护和功能中发挥着关键作用。它对细胞内钙稳态的参与通过其减少 ATP 诱导的钙释放的能力得以体现。CIB2 以单体或同二聚体形式存在，并与各种蛋白质相互作用，包括 WHRN、MYO7A、ITGA2B、ITGA7、TMC1 和 TMC2，这些相互作用通常依赖于钙和镁。

Human

E. coli

N-6*His

O75838-1 (M1-I187)

10518  [NCBI]

MGNKQTIFTEEQLDNYQDCTFFNKKDILKLHSRFYELAPNLVPMDYRKSPIVHVPMSLIIQMPELRENPFKERIVAAFSEDGEGNLTFNDFVDMFSVLCESAPRELKANYAFKIYDFNTDNFICKEDLELTLARLTKSELDEEEVVLVCDKVIEEADLDGDGKLGFADFEDMIAKAPDFLSTFHIRI

Approximately 26 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 200 mM NaCl, 500 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.