1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. CKMT1A 蛋白, Human (sf9, His)

CKMT1A 蛋白, Human (sf9, His)

目录号: HY-P76264
COA 产品使用指南

CKMT1A 是一种肌酸激酶同工酶,在 ATP 和磷酸肌酸之间的磷酸盐可逆转移中发挥关键作用。这种酶的功能对于骨骼肌、心脏、大脑和精子等具有动态能量需求的组织至关重要。CKMT1A 蛋白, Human (sf9, His) 是重组的 CKMT1A 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。CKMT1A 蛋白, Human (sf9, His) 全长 378 个氨基酸,分子量约为 ~43 KDa。

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5 μg ¥550 In-stock
10 μg ¥950 In-stock
50 μg ¥2650 In-stock
100 μg ¥4500 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CKMT1A is a creatine kinase isoenzyme that plays a key role in the reversible transfer of phosphate between ATP and creatine phosphate. The function of this enzyme is critical for tissues with dynamic energy needs, such as skeletal muscle, heart, brain, and sperm. CKMT1A Protein, Human (sf9, His) is the recombinant human-derived CKMT1A protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of CKMT1A Protein, Human (sf9, His) is 378 a.a., with molecular weight of ~43 KDa.

研究背景

CKMT1A 蛋白是一种肌酸激酶同工酶,有助于可逆地催化 ATP 和各种磷原(尤其是磷酸肌酸)之间的磷酸盐转移。这种酶活性对于能量需求大量且波动的组织(包括骨骼肌、心脏、大脑和精子)具有重要意义。肌酸激酶同工酶,包括 CKMT1A,在能量转导中发挥着核心作用,促进能量的有效利用和储存,以响应这些组织的动态代谢需求。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

P12532 (A40-H417)

基因 ID
蛋白结构
N-term
His
CKMT1A (A40-H417)
Accession # P12532
C-term
同用名
Creatine kinase U-type, mitochondrial; Mia-CK; U-MtCK; CKMT; CKMT1B
分子量

Approximately 43 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10% gly.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

CKMT1A Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CKMT1A Protein, Human (sf9, His)
目录号:
HY-P76264
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