Claudin-1 Protein-VLP, Human (HEK293)

The CLDN1 protein is a tight junction component that regulates epithelial permeability. Claudin-1 Protein-VLP, Human (HEK293) is the recombinant human-derived Claudin-1 protein, expressed by HEK293, with tag free.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.

CLDN1 蛋白是紧密连接蛋白家族的成员，作为紧密连接复合物的主要成分，在调节上皮屏障的通透性方面发挥着至关重要的作用。虽然某些密蛋白家族成员有助于形成不可渗透的屏障，但其他成员则调节离子和小分子的渗透性。各种密蛋白家族成员之间的相互作用（通常是共表达的）决定了紧密连接的整体通透性。CLDN1 对于防止小分子通过表皮紧密连接进行细胞旁扩散特别重要，这对于维持皮肤的正常屏障功能和确保水稳态至关重要。有趣的是，虽然 CLDN1 对于角质细胞紧密连接中水屏障的形成似乎是可有可无的，但它可能通过调节其他蛋白质表达水平来间接影响水分流失。此外，CLDN1在肝细胞中充当丙型肝炎病毒（HCV）的共同受体，与CD81形成受体复合物，这对于HCV进入宿主细胞至关重要，并且它还充当登革热病毒的受体。这些不同的作用强调了 CLDN1 在生理屏障功能和病毒感染过程中的重要性。

Human

HEK293

Tag Free

O95832 M1-V211)

9076

Full Length

溶液

Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.