2. 重组蛋白
  3. Receptor Proteins
  4. Claudin-3/CLDN3 Protein-VLP, Human (HEK293)

Claudin-3/CLDN3 Protein-VLP, Human (HEK293)

目录号: HY-P704506
产品使用指南 技术支持

Claudin-3/CLDN3 蛋白在紧密连接中发挥着关键作用，通过不依赖钙的细胞粘附活性消除细胞间隙。它通过与其他 CLDN 成员（包括 CLDN1 和 CLDN2）形成均聚物和杂聚物而表现出多功能性。Claudin-3/CLDN3 Protein-VLP, Human (HEK293) 是重组的 Claudin-3/CLDN3 蛋白，由 HEK293 表达，不带标签。VLPs在HEK293细胞培养中形成，将膜蛋白集中在细胞表面，产生适合免疫和抗体筛选的高浓度蛋白。VLPs免疫原性强，需优化免疫策略，包括抗原剂量、接种方案和佐剂选择。本产品不推荐应用于受配体互作实验和SPR/BLI分析, 因为VLP包膜上还有宿主其他无关膜蛋白, 受配体互作会有很强的背景干扰。SPR/BLI分析则对芯片和实验方案有较高要求，成功率较低。如果需要VLP对照，推荐HY-P705433。

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SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
20 μg ￥7350 1 - 2 周
100 μg ￥14700 1 - 2 周
> 100 μg   询价  

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描述

Claudin-3/CLDN3 proteins play a critical role in tight junctions, eliminating intercellular spaces through calcium-independent cell adhesion activity. It exhibits multifunctionality by forming homo- and heteropolymers with other CLDN members, including CLDN1 and CLDN2. Claudin-3/CLDN3 Protein-VLP, Human (HEK293) is the recombinant human-derived Claudin-3/CLDN3 protein, expressed by HEK293, with tag free.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.
研究背景

Claudin-3/CLDN3 蛋白利用不依赖于钙的细胞粘附活性，在紧密连接内细胞间隙的特异性消除中发挥着至关重要的作用。该蛋白质通过与其他 CLDN 成员形成同聚物和异聚物，包括与 CLDN1 和 CLDN2 同聚物的相互作用，展示了其多功能性。此外，Claudin-3/CLDN3 直接与紧密连接相关蛋白 TJP1/ZO-1、TJP2/ZO-2 和 TJP3/ZO-3 结合，强调其在紧密连接复合物的组装和维护中的不可或缺的作用。形成聚合物并与关键连接成分相互作用的能力凸显了 Claudin-3/CLDN3 在调节细胞粘附和细胞间隙结构完整性方面的重要性。
种属

Human
表达系统

HEK293
标签

Tag Free
蛋白编号

O15551 (M1-V220)
基因 ID

1365
蛋白结构
N-term
CLDN3 (M1-V220)
Accession # O15551
C-term
蛋白长度

Full Length
同用名
C7orf1; Claudin-3; Claudin3; CLD3_HUMAN; CLDN 3; Cldn3; Clostridium perfringens enterotoxin receptor 2; CPE R2; CPE receptor 2; CPE-R 2; CPE-receptor 2; CPETR 2; CPETR2; HRVP 1; HRVP1; Rat ventral prostate 1 like protein; Rat ventral prostate.1 protein homolog; RVP1; Ventral prostate.1 like protein; Ventral prostate.1 protein homolog
糖基化修饰
Yes
性状

溶液.
组分

Supplied as 0.2 μm filtered solution of PBS, Arginine, pH7.4 with trehalose as protectant.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料
产品使用指南 (1213 KB)

Claudin-3/CLDN3 Protein-VLP, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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