Claudin-4/CLDN4 Protein-VLP, Human (HEK293)

Claudin-4/CLDN4 protein-VLP is a channel-forming tight junction protein that promotes paracellular chloride transport in the kidney. It is essential for the reabsorption of filtered chloride in the collecting duct. Claudin-4/CLDN4 Protein-VLP, Human (HEK293) is the recombinant human-derived Claudin-4/CLDN4 protein, expressed by HEK293, with tag free.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.

Claudin-4 (CLDN4) 蛋白是一种通道形成紧密连接蛋白，是肾脏中细胞旁氯离子转运的关键介质，尤其影响集合管中细胞旁氯离子重吸收的关键过程。在紧密连接内，包括 CLDN4 在内的紧密连接蛋白在细胞间隙的特异性消除中发挥着至关重要的作用，表现出不依赖于钙的细胞粘附活性。该蛋白与 TJP1/ZO-1、TJP2/ZO-2 和 TJP3/ZO-3 等关键紧密连接成分建立直接相互作用。此外，CLDN4 与 EPHA2 结合，其中 EPHA2 介导的 CLDN4 磷酸化可能有助于紧密连接的调节。值得注意的是，CLDN4 还与其他密蛋白家族成员（例如 CLDN1 和 CLDN8）相互作用，进一步强调了它参与复杂的紧密连接网络。

Human

HEK293

Tag Free

O14493 (M1-V209)

1364

Full Length

溶液.

Supplied as 0.2 μm filtered solution of PBS, Arginine, pH7.4 with trehalose as protectant.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.