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  3. Claudin-9/CLDN9 Protein-VLP, Human (HEK293)

Claudin-9/CLDN9 Protein-VLP, Human (HEK293)

目录号: HY-P703890
产品使用指南 技术支持

Claudin-9/CLDN9 蛋白-VLP 通过不依赖钙的细胞粘附活性消除细胞间隙,在紧密连接中发挥着至关重要的作用。在微生物感染中,它充当促进丙型肝炎病毒(HCV)进入肝细胞的受体。Claudin-9/CLDN9 Protein-VLP, Human (HEK293) 是重组的 Claudin-9/CLDN9 蛋白,由 HEK293 表达,不带标签。VLPs在HEK293细胞培养中形成,将膜蛋白集中在细胞表面,产生适合免疫和抗体筛选的高浓度蛋白。VLPs免疫原性强,需优化免疫策略,包括抗原剂量、接种方案和佐剂选择。本产品不推荐应用于受配体互作实验和SPR/BLI分析, 因为VLP包膜上还有宿主其他无关膜蛋白, 受配体互作会有很强的背景干扰. SPR/BLI分析则对芯片和实验方案有较高要求,成功率较低. 如果需要VLP对照,推荐HY-P701236。标签只能在变性条件下检测到。

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     可免费申领三个不同产品的试用装。

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描述

Claudin-9/CLDN9 protein-VLP plays a crucial role in tight junctions by eliminating intercellular spaces through calcium-independent cell adhesion activity. In microbial infections, it acts as a receptor that facilitates the entry of hepatitis C virus (HCV) into hepatocytes. Claudin-9/CLDN9 Protein-VLP, Human (HEK293) is the recombinant human-derived Claudin-9/CLDN9 protein, expressed by HEK293, with tag free.It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.

研究背景

Claudin-9/CLDN9 蛋白-VLP 利用不依赖于钙的细胞粘附活性,在紧密连接内细胞间隙的特异性消除中发挥着关键作用。值得注意的是,在微生物感染的情况下,这种蛋白质充当促进丙型肝炎病毒(HCV)进入肝细胞的受体。Claudin-9/CLDN9 的双重功能强调了其在调节紧密连接处的细胞粘附方面的重要性,有助于维持组织完整性,同时也作为 HCV 的关键切入点,强调了其在病毒感染过程中的相关性。

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

O95484 (M1-V217)

基因 ID

9080

蛋白长度

Full Length

中文名
Claudin-9 蛋白/CLDN9 蛋白-病毒样颗粒
同用名
Claudin-9; CLD9
预测分子量
22.9 kDa
内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Claudin-9/CLDN9 Protein-VLP, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Claudin-9/CLDN9 Protein-VLP, Human (HEK293)
目录号:
HY-P703890
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