CLEC4E 蛋白, Human (HEK293, His)

The CLEC4E protein is a calcium-dependent lectin that functions as a pattern recognition receptor in the immune system. It detects and binds DAMPs and PAMPs, including α-mannose residues on mycobacterial TDM and fungi. CLEC4E Protein, Human (HEK293, His) is the recombinant human-derived CLEC4E protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CLEC4E Protein, Human (HEK293, His) is 179 a.a., with molecular weight of ~26.0 kDa.

CLEC4E 蛋白是一种钙依赖性凝集素，在先天免疫系统中充当模式识别受体 (PRR)。它负责检测并结合细菌和真菌中发现的异常自身的损伤相关分子模式 (DAMP) 和病原体相关分子模式 (PAMP)。值得注意的是，它可以识别分枝杆菌海藻糖 6,6'-二霉菌酸酯 (TDM)，这是一种具有有效免疫调节功能的糖脂。在骨髓细胞中，CLEC4E 与信号转导接头 Fc 受体 γ 链/FCER1G 相互作用，形成功能复合物。TDM 与该受体复合物的结合会触发 FCER1G 的免疫受体酪氨酸激活基序 (ITAM) 的磷酸化，从而激活 SYK、CARD9 和 NF-kappa-B。这最终会驱动抗原呈递细胞的成熟，并形成 T 细胞针对效应 T 辅助细胞 1 和 T 辅助细胞 17 细胞亚型的抗原特异性启动。CLEC4E 还识别病原真菌（例如马拉色菌）上的 α-甘露糖残基，并介导巨噬细胞激活。此外，CLEC4E 通过识别非稳态细胞死亡过程中释放的 DAMP 来实现对受损自身的免疫感应，从而促进炎症细胞浸润到受损组织。CLEC4E 可以单体或同二聚体形式存在，并直接与 FCER1G 相互作用形成功能复合物。或者，它充当 CLEC4D 和 FCER1G 之间交互的桥梁。此外，CLEC4E可以直接与坏死细胞释放的SAP130核蛋白相互作用。

Human

HEK293

C-6*His

Q9ULY5 (R41-L219)

26253  [NCBI]

RCVVTFRIFQTCDEKKFQLPENFTELSCYNYGSGSVKNCCPLNWEYFQSSCYFFSTDTISWALSLKNCSAMGAHLVVINSQEEQEFLSYKKPKMREFFIGLSDQVVEGQWQWVDGTPLTKSLSFWDVGEPNNIATLEDCATMRDSSNPRQNWNDVTCFLNYFRICEMVGINPLNKGKSL

Approximately 26.0 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.