1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. CLM9/CD300g 蛋白, Rat (HEK293, His)

CLM9/CD300g 蛋白, Rat (HEK293, His)

目录号: HY-P74246
COA 产品使用指南

CLM9/CD300g 蛋白被称为受体,被认为参与介导 L-选择素依赖性淋巴细胞滚动。该蛋白与 SELL(L-选择素配体)表现出钙依赖性结合,表明其能够与淋巴细胞相互作用并可能影响淋巴细胞相关过程。CLM9/CD300g 蛋白, Rat (HEK293, His) 是重组的 CLM9/CD300g 蛋白,由 HEK293 表达,带有 C-His 标签。CLM9/CD300g 蛋白, Rat (HEK293, His) 全长 160 个氨基酸,分子量约为 25-30 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The CLM9/CD300g protein is known as a receptor and is thought to be involved in mediating L-selectin-dependent lymphocyte rolling. This protein exhibits calcium-dependent binding to SELL (L-selectin ligand), indicating its ability to interact with lymphocytes and potentially influence lymphocyte-related processes. CLM9/CD300g Protein, Rat (HEK293, His) is the recombinant rat-derived CLM9/CD300g protein, expressed by HEK293 , with C-His labeled tag. The total length of CLM9/CD300g Protein, Rat (HEK293, His) is 160 a.a., with molecular weight of 25-30 kDa.

研究背景

CLM9/CD300g 蛋白被称为受体,被认为参与介导依赖于 L-选择素的淋巴细胞滚动。该蛋白与 SELL(L-选择素配体)表现出钙依赖性结合,表明其能够与淋巴细胞相互作用并可能影响淋巴细胞相关过程。CLM9/CD300g 蛋白的存在意味着其在细胞粘附和迁移中的重要性,特别是在淋巴细胞功能方面。需要进一步的研究来充分了解这种蛋白质的精确机制和功能,但它参与淋巴细胞滚动和相互作用强调了它在免疫反应和淋巴细胞介导过程中的潜在重要性。

生物活性

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 1.244 μg/mL. Corresponding to a specific activity is 803.859 U/mg.

种属

Rat

表达系统

HEK293

标签

C-6*His

蛋白编号

XP_002724611.1 (L19-R160)

基因 ID

684984  [NCBI]

蛋白结构
N-term
CD300LG (M1-R160)
Accession # XP_002724611
His
C-term
同用名
CMRF35-Like Molecule 9; CLM-9; TREM-4; CD300g; CD300LG
氨基酸序列

LTGPKEISGFEGDTVSLRCTYKKEMKVHRKYWCRQGGILLSRCGDTVYTNQDQEVTRGKMSIRDSLQDLWVTVTMRDLTLKDSGKYWCGIDRLGRDESFEVKLIVFPGSSRPVIWPPLTTPKDSRAVTSSVSKSSVSIPMVR

分子量

Approximately 25-30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CLM9/CD300g Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CLM9/CD300g Protein, Rat (HEK293, His)
目录号:
HY-P74246
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