2. 重组蛋白
  3. Viral Proteins
  4. Bacterial/Fungal Proteins
  5. Clumping factor A 蛋白, S. aureus (P.pastoris, His)

Clumping factor A 蛋白, S. aureus (P.pastoris, His)

目录号: HY-P71829
产品使用指南

凝集因子 A 蛋白有利于细菌附着在人纤维蛋白原 γ 链上，从而促进细菌凝块的形成。它是一种细胞表面相关蛋白，在细菌毒力中发挥作用。Clumping factor A 蛋白, S. aureus (P.pastoris, His) 是重组的 凝集因子 A 蛋白，由 P. pastoris 表达，带有 N-His 标签。Clumping factor A 蛋白, S. aureus (P.pastoris, His) 全长 331 个氨基酸，分子量约为 ~38.5 kDa。

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描述

Clumping factor A protein facilitates bacterial attachment to human fibrinogen gamma-chain, promoting the formation of bacterial clumps. It is a cell surface-associated protein and plays a role in bacterial virulence. Clumping factor A Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Clumping factor A protein, expressed by P. pastoris , with N-His labeled tag. The total length of Clumping factor A Protein, S. aureus (P.pastoris, His) is 331 a.a., with molecular weight of ~38.5 kDa.
研究背景

聚集因子 A 蛋白是一种细胞表面相关蛋白，以其在细菌毒力中的作用而闻名。它特别促进细菌附着在人纤维蛋白原的伽玛链上，促进细菌团块的形成（通过相似性）。
种属

Staphylococcus aureus
表达系统

P. pastoris
标签

N-His
蛋白编号

Q5HHM8 (229G-559E)
基因 ID

/
蛋白结构
N-term
His
Clumping factor A (229G-559E)
Accession # Q5HHM8
C-term
同用名
clfA; Fibrinogen receptor A; Fibrinogen-binding protein A
氨基酸序列

GTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLTMPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFVNPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRSTLYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPE

分子量

Approximately 38.5 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Clumping factor A Protein, S. aureus (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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