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  3. Clusterin/APOJ 蛋白, Human (HEK293, Fc-His)

Clusterin/APOJ 蛋白, Human (HEK293, Fc-His)

目录号: HY-P7535
COA 产品使用指南

Clusterin/APOJ 蛋白, Human (HEK293, Fc-His)在 HEK293 细胞中表达,N 端带有 Fc 片段和 His 标签。Clusterin/APOJ 蛋白 参与许多对脂质运输,血管平滑肌细胞分化很重要的生理过程,并具有用于动脉粥样硬化的潜力。

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规格 价格 是否有货 数量
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5 μg ¥820 In-stock
10 μg ¥1400 In-stock
50 μg ¥3600 In-stock
100 μg   询价  

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Clusterin/APOJ Protein, Human (HEK293, Fc-His) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Clusterin/APOJ Protein, Human (HEK293, Fc-His) expresses in HEK293 with an Fc fragment and a His tag at the N-terminus. Apolipoprotein J (ApoJ) is involved in numerous physiological process important for lipid transportation, vascular smooth muscle cell differentiation, and has the potential for atherosclerosis[1].

研究背景

载脂蛋白J(apoJ),也称为簇蛋白(CLU),与血液中HDL中的脂质和apoA1紧密相关,通过大量研究已被提议作为此类生物标志物。在缓解动脉粥样硬化的过程中,apoJ可以促进巨噬细胞泡沫细胞输出胆固醇和磷脂,并通过与许多已知的炎症蛋白相互作用而发挥细胞保护和抗炎作用,这可以预测临床心血管事件的发生,并可能实际上发挥作用C 反应蛋白、对氧磷酶和瘦素等在介导动脉粥样硬化疾病中的因果作用[1]

生物活性

Measured by its ability to induce clustering of Caki-2 human clear cell carcinoma epithelial cells.

种属

Human

表达系统

HEK293

标签

C-6*His;C-Fc

蛋白编号

P10909 (D23-E449)

基因 ID
蛋白结构
N-term
Clusterin (D23-E449)
Accession # P10909
hFc-6*His
C-term
同用名
rHuApolipoprotein J, C-Fc-His; ApoJ; Clusterin; CLU; CLI; KUB1; Apolipoprotein J
氨基酸序列

DQTVSDNELQEMSNQGSKYVNKEIQNAVNGVKQIKTLIEKTNEERKTLLSNLEEAKKKKEDALNETRESETKLKELPGVCNETMMALWEECKPCLKQTCMKFYARVCRSGSGLVGRQLEEFLNQSSPFYFWMNGDRIDSLLENDRQQTHMLDVMQDHFSRASSIIDELFQDRFFTREPQDTYHYLPFSLPHRRPHFFFPKSRIVRSLMPFSPYEPLNFHAMFQPFLEMIHEAQQAMDIHFHSPAFQHPPTEFIREGDDDRTVCREIRHNSTGCLRMKDQCDKCREILSVDCSTNNPSQAKLRRELDESLQVAERLTRKYNELLKSYQWKMLNTSSLLEQLNEQFNWVSRLANLTQGEDQYYLRVTTVASHTSDSDVPSGVTEVVVKLFDSDPITVTVPVEVSRKNPKFMETVAEKALQEYRKKHREE

分子量

Approximately (35-40) & (61-79) & 90 kD

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Clusterin/APOJ Protein, Human (HEK293, Fc-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Clusterin/APOJ Protein, Human (HEK293, Fc-His)
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