CMKLR1 蛋白, Human (Cell-Free, His)

CMKLR1 protein is a receptor for chemerin/RARRES2 and resolvin E1, activating G protein and β-arrestin pathways when RARRES2 interacts. This triggers a cascade of reactions including calcium mobilization, MAP kinase phosphorylation, and PI3K activation, leading to various effects including reduced immune response, adipogenesis, and angiogenesis. CMKLR1 Protein, Human (Cell-Free, His) is the recombinant human-derived CMKLR1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of CMKLR1 Protein, Human (Cell-Free, His) is 373 a.a., with molecular weight of 45.1 kDa.

CMKLR1 蛋白充当趋化剂脂肪因子 chemerin/RARRES2 和 omega-3 脂肪酸衍生分子 resolvin E1 的受体。与 RARRES2 相互作用后，CMKLR1 激活 G 蛋白 G(i)/G(o) 和 β-arrestin 通路，通过第二信使通路触发细胞反应，如细胞内钙动员、MAP 激酶 (MAPK1/MAPK3)、TYRO3、MAPK14/ 磷酸化P38MAPK 和 PI3K。这些级联反应会产生多功能效应，包括减少免疫反应、增强脂肪生成和血管生成。另一方面，Resolvin E1 通过减少 MAPK1/3 (ERK1/2) 和 NF-kappa-B 的激活来下调巨噬细胞中细胞因子的产生。CMKLR1还在脂肪生成和脂肪细胞代谢中发挥积极的调节作用。在微生物感染的情况下，CMKLR1 充当多种 SIV 毒株（SIVMAC316、SIVMAC239、SIVMACL7E-FR 和 SIVSM62A）以及主要 HIV-1 毒株（92UG024-2）的共受体。

Human

E. coli Cell-free

N-10*His

Q99788 (M1-L373)

1240

MRMEDEDYNTSISYGDEYPDYLDSIVVLEDLSPLEARVTRIFLVVVYSIVCFLGILGNGLVIIIATFKMKKTVNMVWFLNLAVADFLFNVFLPIHITYAAMDYHWVFGTAMCKISNFLLIHNMFTSVFLLTIISSDRCISVLLPVWSQNHRSVRLAYMACMVIWVLAFFLSSPSLVFRDTANLHGKISCFNNFSLSTPGSSSWPTHSQMDPVGYSRHMVVTVTRFLCGFLVPVLIITACYLTIVCKLQRNRLAKTKKPFKIIVTIIITFFLCWCPYHTLNLLELHHTAMPGSVFSLGLPLATALAIANSCMNPILYVFMGQDFKKFKVALFSRLVNALSEDTGHSSYPSHRSFTKMSSMNERTSMNERETGML

45.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.