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  3. CNPY3/PRAT4A 蛋白, Mouse (HEK293, His)

CNPY3/PRAT4A 蛋白, Mouse (HEK293, His)

目录号: HY-P76274
产品使用指南

CNPY3/PRAT4A 蛋白是 HSP90B1 的 Toll 样受体 (TLR) 特异性共伴侣,对于 TLR(不包括 TLR3)从内质网正确折叠和退出至关重要。CNPY3/PRAT4A 对于先天性和适应性免疫反应至关重要,与 HSP90B1 相互作用,并在 ATP 存在时被破坏。CNPY3/PRAT4A 蛋白, Mouse (HEK293, His) 是重组的 CNPY3/PRAT4A 蛋白,由 HEK293 表达,带有 C-His 标签。CNPY3/PRAT4A 蛋白, Mouse (HEK293, His) 全长 272 个氨基酸,分子量约为 ~28.6 KDa。

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描述

The CNPY3/PRAT4A protein is a Toll-like receptor (TLR)-specific co-chaperone of HSP90B1 and is essential for the correct folding and exit of TLRs (excluding TLR3) from the endoplasmic reticulum. CNPY3/PRAT4A is critical for innate and adaptive immune responses, interacts with HSP90B1, and is destroyed in the presence of ATP. CNPY3/PRAT4A Protein, Mouse (HEK293, His) is the recombinant mouse-derived CNPY3/PRAT4A protein, expressed by HEK293 , with C-His labeled tag. The total length of CNPY3/PRAT4A Protein, Mouse (HEK293, His) is 272 a.a., with molecular weight of ~28.6 KDa.

研究背景

CNPY3/PRAT4A 蛋白作为 HSP90B1 的 Toll 样受体 (TLR) 特异性共伴侣,在 TLR(TLR3 除外)的正确折叠中发挥着至关重要的作用,从而控制它们从内质网的退出。这种蛋白质的参与对于先天性和适应性免疫反应都至关重要。CNPY3/PRAT4A 与 HSP90B1 相互作用,并且这种相互作用在 ATP 存在时被破坏。此外,它与各种 TLR 结合,包括 TLR1、TLR2、TLR4 和 TLR9,其中与 TLR4 的相互作用最强。CNPY3/PRAT4A 和 TLR 之间错综复杂的相互作用强调了其在调节免疫反应中的重要性,并强调了其在控制 TLR 正确折叠和运输的细胞机制中作为辅助伴侣的作用。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q9DAU1 (M1-P272)

基因 ID

72029  [NCBI]

蛋白结构
N-term
CNPY3 (M1-P272)
Accession # Q9DAU1
His
C-term
同用名
Protein canopy homolog 3; Protein associated with Tlr4; PRAT4A; TNRC5
分子量

Approximately 28.6 kDa.

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CNPY3/PRAT4A Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CNPY3/PRAT4A Protein, Mouse (HEK293, His)
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