CNR1 Protein-VLP, Human (T210A, HEK293)

CNR1 protein-VLP is a G-protein-coupled receptor for endogenous and phytocannabinoids that mediates multiple effects on food intake, memory, and pain. It regulates mitochondrial respiration, inhibits ROS formation, and induces gene expression. CNR1 Protein-VLP, Human (T210A, HEK293) is the recombinant human-derived CNR1 protein, expressed by HEK293, with tag free.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.

CNR1 蛋白-VLP 充当内源性大麻素 (eCB) 的 G 蛋白偶联受体，包括 N-花生四烯酰乙醇酰胺（anandamide 或 AEA）和 2-花生四烯酰甘油 (2-AG)，以及植物大麻素，如 delta(9)-四氢大麻酚（四氢大麻酚）。它介导一系列大麻素引起的对食物摄入、记忆、胃肠道运动、僵直、行走活动、焦虑和慢性疼痛的影响，通常涉及环磷酸腺苷的减少。在下丘脑中，它对线粒体呼吸表现出双重作用，通过 G 蛋白 α-i 蛋白激活和随后抑制线粒体可溶性腺苷酸环化酶，在低剂量时增加呼吸，在高剂量时减少呼吸。此外，CNR1 抑制瘦素诱导的活性氧 (ROS) 形成，介导大麻素诱导的 SREBF1 和 FASN 基因表达增加，并驱动下丘脑 POMC 神经元释放促进食欲的 β-内啡肽。在海马体中，它响应大麻素调节细胞呼吸和能量产生，并参与大麻素依赖性过程，如去极化诱导的抑制抑制 (DSI)。CNR1 还抑制颈上神经节和脑血管平滑肌细胞中的电压门控 Ca(2+) 通道，导致血管舒张和血管张力降低。它诱导脂肪细胞中瘦素的产生，减少肾脏中 LRP2 介导的瘦素清除率，并调节脂肪组织和肝脏中的脂质代谢。此外，CNR1 外周调节能量代谢，并且在高碳水化合物饮食引起的肥胖中，可能影响横纹肌中线粒体二氢硫辛酰脱氢酶/DLD 表达和选定的葡萄糖/丙酮酸代谢酶。作为对大麻素 anandamide 的反应，CNR1 在巨噬细胞中引发促炎反应，可能导致 2 型糖尿病的进展和胰腺 β 细胞的损失。值得注意的是，CNR1 结合 2-花生四烯酰甘油 (2-AG) 和花生四烯酸乙醇胺。

Human

HEK293

Tag Free

P21554 (M1-L472, T210A)

1268

溶液.

Supplied as 0.2 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.