1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins
  3. Neurotrophic Factors Cytokine Receptors
  4. Ciliary Neurotrophic Factor Receptor (CNTFR)
  5. CNTFR alpha 蛋白, Rat (sf9, His)

CNTFR alpha 蛋白, Rat (sf9, His)

目录号: HY-P76276
产品使用指南

CNTFR α 蛋白对于与睫状神经营养因子 (CNTF) 的结合至关重要,通过其 α 亚基确保受体特异性。它与 LIFR 和 IL6ST 形成异源三聚体复合物,形成功能性受体。CNTFR α 还与 CLCF1/CLC 和 CRLF1/CLF-1 异二聚体相互作用。无论是单独使用还是与 CLCF1-CRLF1 异二聚体一起使用,CNTFR α 都会与 SORL1 结合,通过这种相互作用潜在地促进内化和溶酶体降解。CNTFR alpha 蛋白, Rat (sf9, His) 是重组的 CNTFR α 蛋白,由 Sf9 昆虫细胞表达,带有 C-His 标签。CNTFR alpha 蛋白, Rat (sf9, His) 全长 346 个氨基酸,分子量约为 ~48 KDa。

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描述

CNTFR alpha protein is pivotal in binding to ciliary neurotrophic factor (CNTF), ensuring receptor specificity through its alpha subunit. It forms a heterotrimeric complex with LIFR and IL6ST, contributing to a functional receptor. CNTFR alpha also interacts with the CLCF1/CLC and CRLF1/CLF-1 heterodimer. Whether alone or with the CLCF1-CRLF1 heterodimer, CNTFR alpha engages with SORL1, potentially facilitating internalization and lysosomal degradation through this interaction. CNTFR alpha Protein, Rat (sf9, His) is the recombinant rat-derived CNTFR alpha protein, expressed by Sf9 insect cells, with C-His labeled tag. The total length of CNTFR alpha Protein, Rat (sf9, His) is 346 a.a., with molecular weight of ~48 KDa.

研究背景

CNTFR α 蛋白在与睫状神经营养因子 (CNTF) 的结合中发挥着至关重要的作用,其 α 亚基提供受体特异性。它与 LIFR 和 IL6ST 形成异三聚体复合物,有助于功能性受体的组装。此外,CNTFR α 与由 CLCF1/CLC 和 CRLF1/CLF-1 组成的异二聚神经营养细胞因子相互作用。值得注意的是,无论是单独还是与 CLCF1-CRLF1 异二聚体结合,CNTFR α 都会与 SORL1 结合,通过这种相互作用潜在地促进内化和溶酶体降解。

种属

Rat

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q08406 (M1-P346)

基因 ID

313173  [NCBI]

蛋白结构
N-term
CNTFR alpha (M1-P346)
Accession # Q08406
His
C-term
同用名
Ciliary neurotrophic factor receptor subunit alpha; CNTFR-alpha
分子量

Approximately 48 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CNTFR alpha Protein, Rat (sf9, His)
目录号:
HY-P76276
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