Complement C3 蛋白, Human (HEK293, hFc)

Complement C3/C3a proteins play a key role in initiating the complement system through processing by C3 convertase in the classical and alternative pathways. Upon activation, C3b covalently binds to the surface, while C3a (anaphylatoxin produced by proteolysis of C3) mediates local inflammation. Complement C3 Protein, Human (HEK293, hFc) is the recombinant human-derived Complement C3 protein, expressed by HEK293, with C-hFc labeled tag.

C3 在补体系统的启动中发挥着关键作用，C3 转化酶对其进行的加工构成了经典补体途径和替代补体途径中的中心反应。激活后，C3b 能够通过其反应性硫酯共价结合细胞表面碳水化合物或免疫聚集体。同时，源自补体 C3 蛋白水解降解的过敏毒素 C3a 充当局部炎症过程的介质。在慢性炎症的情况下，C3a 充当中性粒细胞的化学引诱剂，诱导平滑肌收缩，增加血管通透性，并触发肥大细胞和嗜碱性白细胞释放组胺。

Human

HEK293

C-hFc

P01024 (S23-N1663)

718

Approximately 75-80 kDa,160-180 kDa & 180 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.