1. 重组蛋白
  2. Complement System
  3. Complement Component 5
  4. Complement Component 5a
  5. Complement C5/C5a 蛋白, Human (His)

Complement C5/C5a 蛋白, Human (His)

目录号: HY-P700959
产品使用指南

补体 C5 被 C5 转化酶激活,诱导 C5-C9 组装形成膜攻击复合物。C5b 上的瞬时 C6 结合位点对于裂解复合物的形成至关重要。Complement C5/C5a 蛋白, Human (His) 是重组的 补体 C5/C5a 蛋白,由 E. coli 表达,带有 N-His 标签。Complement C5/C5a 蛋白, Human (His) 全长 74 个氨基酸,分子量为 13-14 kDa。

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描述

Complement C5 is activated by C5 convertase, inducing C5-C9 assembly to form the membrane attack complex. The transient C6 binding site on C5b is critical for the formation of the cleavage complex. Complement C5/C5a Protein, Human (His) is the recombinant human-derived Complement C5/C5a protein, expressed by E. coli , with N-His labeled tag. The total length of Complement C5/C5a Protein, Human (His) is 74 a.a., with molecular weight of 13-14 kDa.

研究背景

被 C5 转化酶激活后,补体 C5 启动后期补体成分 C5-C9 的自发组装,形成膜攻击复合物。C6 在 C5b 上的瞬时结合位点对于裂解复合物的基础至关重要。补体 C5 的蛋白水解降解产生 C5a 过敏毒素,它是局部炎症过程的介质。C5a 与其受体 C5AR1 相互作用,引发多种反应,例如细胞内钙释放、平滑肌收缩、血管通透性增加以及肥大细胞和嗜碱性白细胞释放组胺。作为一种有效的趋化因子,C5a 刺激多形核白细胞的运动并引导它们向炎症部位迁移,从而有助于协调免疫反应。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P01031 (T678-R751)

基因 ID

727  [NCBI]

蛋白结构
N-term
His
C5a (T678-R751)
Accession # P01031
C-term
同用名
C5a; Complement C5; C5; CPAMD4; ECLZB
分子量

13-14 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Complement C5/C5a Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Complement C5/C5a Protein, Human (His)
目录号:
HY-P700959
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