2. 重组蛋白
  3. Complement System Biotinylated Proteins
  4. Complement Component 5
  5. Complement Component 5a
  6. Complement C5/C5a 蛋白, Mouse (Biotinylated)

Complement C5/C5a 蛋白, Mouse (Biotinylated)

目录号: HY-P77540
产品使用指南

补体 C5/C5a 蛋白激活晚期补体成分，形成膜攻击复合物 C5-C9。C5b 结合 C6，启动裂解复合物组装。C5a 由 C5 降解产生，作为过敏毒素，诱发局部炎症。C5a 刺激细胞内钙释放、平滑肌收缩、血管通透性、组胺释放，并充当趋化因子，引导白细胞迁移。Complement C5/C5a 蛋白, Mouse (Biotinylated) 是重组的 补充 C5/C5a 蛋白，由 E. coli 表达，不带标签。Complement C5/C5a 蛋白, Mouse (Biotinylated) 全长 77 个氨基酸，分子量约为 ~9 KDa。

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100 μg   询价  

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描述

Complement C5/C5a Protein activates late complement components to form the membrane attack complex, C5-C9. C5b binds C6, initiating lytic complex assembly. C5a, produced by C5 degradation, acts as an anaphylatoxin, inducing local inflammation. C5a stimulates intracellular calcium release, smooth muscle contraction, vascular permeability, histamine release, and acts as a chemokine, guiding leukocyte migration. Complement C5/C5a Protein, Mouse (Biotinylated) is the recombinant mouse-derived Complement C5/C5a protein, expressed by E. coli, with tag free. The total length of Complement C5/C5a Protein, Mouse (Biotinylated) is 77 a.a., with molecular weight of ~9 KDa.
研究背景

补体 C5/C5a 蛋白被 C5 转化酶激活时，会触发后期补体成分自发组装成膜攻击复合物 C5-C9。C5b 形成 C6 的瞬时结合位点，形成裂解复合物组装的基础。同时，通过补体 C5 的蛋白水解降解产生的 C5a，充当介导局部炎症过程的过敏毒素。它与受体 C5AR1 的结合诱导各种反应，例如细胞内钙释放、平滑肌收缩、血管通透性增加以及肥大细胞和嗜碱性白细胞释放组胺。此外，C5a 作为一种有效的趋化因子，刺激多形核白细胞的运动并引导它们向发炎区域迁移。
种属

Mouse
表达系统

E. coli
标签

Tag Free
蛋白编号

P06684 (N679-R755)
基因 ID

15139  [NCBI]
蛋白结构
N-term
C5a (N679-R755)
Accession # P06684
C-term
同用名
Complement C5; Hemolytic Complement; C5a
分子量

Approximately 9 kDa.
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Complement C5/C5a Protein, Mouse (Biotinylated) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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