Complement Factor B/CFB 蛋白, Human (HEK293, His)

Complement Factor B/CFB Protein, Human (HEK293, His) is the recombinant human-derived Complement Factor B/CFB protein, expressed by HEK293, with C-6*His labeled tag.

Complement factor B (CFB) 是补体系统替代途径的一部分可被因子 D 切割成两个片段： Ba 和 Bb。Bb 是一种丝氨酸蛋白酶，然后与补体因子 3b 结合产生 C3 或 C5 转化酶，还与预活化 b 淋巴细胞的增殖和分化、外周血单核细胞的快速扩散、淋巴细胞胚发生的刺激和红细胞的溶解有关。Ba抑制预激活 b 淋巴细胞的增殖。补体替代通路 (AP) 的激活被认为在免疫球蛋白 A 肾病 (IgAN) 中起重要作用。CFB 可抑制 AP 的活性。CFB 的表达与 T 细胞 CD8、巨噬细胞 M1、浆细胞、 T 细胞 CD4 记忆激活、T 细胞滤泡辅助和 T 细胞调节 (Tregs) 呈正相关，与 TME 中嗜酸性粒细胞、巨噬细胞 M0、巨噬细胞 M2、肥大细胞静息、T 细胞 CD4 记忆静息呈负相关。CFB 调节细胞衰老并与胰腺癌预后不良相关。

Human

HEK293

C-6*His

AAH04143.1 (T26-L764)

629  [NCBI]

Partial

TPWSLARPQGSCSLEGVEIKGGSFRLLQEGQALEYVCPSGFYPYPVQTRTCRSTGSWSTLKTQDQKTVRKAECRAIHCPRPHDFENGEYWPRSPYYNVSDEISFHCYDGYTLRGSANRTCQVNGRWSGQTAICDNGAGYCSNPGIPIGTRKVGSQYRLEDSVTYHCSRGLTLRGSQRRTCQEGGSWSGTEPSCQDSFMYDTPQEVAEAFLSSLTETIEGVDAEDGHGPGEQQKRKIVLDPSGSMNIYLVLDGSDSIGASNFTGAKKCLVNLIEKVASYGVKPRYGLVTYATYPKIWVKVSEADSSNADWVTKQLNEINYEDHKLKSGTNTKKALQAVYSMMSWPDDVPPEGWNRTRHVIILMTDGLHNMGGDPITVIDEIRDLLYIGKDRKNPREDYLDVYVFGVGPLVNQVNINALASKKDNEQHVFKVKDMENLEDVFYQMIDESQSLSLCGMVWEHRKGTDYHKQPWQAKISVIRPSKGHESCMGAVVSEYFVLTAAHCFTVDDKEHSIKVSVGGEKRDLEIEVVLFHPNYNINGKKEAGIPEFYDYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLGFL

90-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.