1. 重组蛋白
  2. Complement System
  3. Complement Regulatory Proteins
  4. Factor D
  5. Complement Factor D/Adipsin 蛋白, Human (HEK293, His)

Complement Factor D/Adipsin 蛋白, Human (HEK293, His)

目录号: HY-P7861
COA 产品使用指南

补体因子 D,也称为 Adipsin,在补体系统的旁路途径中起着至关重要的作用,当与因子 C3b 复合时,它会裂解因子 B。这种裂解会激活 C3bbb 复合物,将其转化为旁路途径的 C3 转化酶。Complement Factor D/Adipsin 蛋白, Human (HEK293, His) 是重组的 补体因子 D/脂肪素蛋白,由 HEK293 表达,带有 C-10*His 标签。Complement Factor D/Adipsin 蛋白, Human (HEK293, His) 全长 228 个氨基酸,分子量约为 ~28.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥1500 In-stock
50 μg ¥4300 In-stock
100 μg   询价  

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描述

Complement factor D, also known as Adipsin, plays a crucial role in the alternative pathway of the complement system and when complexed with factor C3b, it cleaves factor B. This cleavage activates the C3bbb complex, converting it to the C3 convertase of the alternative pathway. Complement Factor D/Adipsin Protein, Human (HEK293, His) is the recombinant human-derived Complement Factor D/Adipsin protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Complement Factor D/Adipsin Protein, Human (HEK293, His) is 228 a.a., with molecular weight of ~28.0 kDa.

研究背景

补体因子 D,也称为 Adipsin,是补体系统替代途径中的重要组成部分。因子 D 的功能与经典途径中的 C1 类似,在 C3 转化酶的激活中发挥着关键作用。具体而言,当因子 B 与因子 C3b 复合时,因子 D 会裂解因子 B。这种酶活性导致活性 C3bbb 复合物的生成,该复合物充当替代途径中的 C3 转化酶。D 因子与 C1s 的同源功能强调了其在启动补体级联中的重要性,强调了其在免疫反应中的作用。

种属

Human

表达系统

HEK293

标签

C-10*His

蛋白编号

P00746 (I26-A253)

基因 ID
蛋白结构
N-term
CFD (I26-A253)
Accession # P00746
10*His
C-term
同用名
rHuComplement Factor D/Adipsin, His; Complement factor D; CFD; Adipsin; C3 convertase activator; Properdin factor D; DF; PFD
氨基酸序列

ILGGREAEAHARPYMASVQLNGAHLCGGVLVAEQWVLSAAHCLEDAADGKVQVLLGAHSLSQPEPSKRLYDVLRAVPHPDSQPDTIDHDLLLLQLSEKATLGPAVRPLPWQRVDRDVAPGTLCDVAGWGIVNHAGRRPDSLQHVLLPVLDRATCNRRTHHDGAITERLMCAESNRRDSCKGDSGGPLVCGGVLEGVVTSGSRVCGNRKKPGIYTRVASYAAWIDSVLA

分子量

Approximately 28.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Complement Factor D/Adipsin Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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