2. 重组蛋白
  3. Complement System
  4. Complement Regulatory Proteins
  5. Factor D
  6. Complement Factor D/Adipsin 蛋白, Mouse (HEK293, Fc)

Complement Factor D/Adipsin 蛋白, Mouse (HEK293, Fc)

目录号: HY-P78273
产品使用指南

补体因子 D/Adipsin 蛋白可裂解与因子 C3b 复合的因子 B，激活 C3bbb 复合物，在替代途径中形成 C3 转化酶，类似于经典途径中的 C1。Complement Factor D/Adipsin 蛋白, Mouse (HEK293, Fc) 是重组的 补体因子 D/脂肪素蛋白，由 HEK293 表达，带有 C-hFc 标签。Complement Factor D/Adipsin 蛋白, Mouse (HEK293, Fc) 全长 234 个氨基酸，分子量为 60-70 kDa。

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100 μg   询价  

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描述

Complement Factor D/Adipsin Protein cleaves factor B in complex with factor C3b, activating the C3bbb complex to form the C3 convertase in the alternate pathway, analogous to C1s in the classical pathway. Complement Factor D/Adipsin Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Complement Factor D/Adipsin protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Complement Factor D/Adipsin Protein, Mouse (HEK293, Fc) is 234 a.a., with molecular weight of 60-70 kDa.
研究背景

补体因子 D/Adipsin 蛋白通过裂解因子 B 发挥作用，特别是当它与因子 C3b 复合时。这种裂解激活 C3bbb 复合物，将其转化为替代途径的 C3 转化酶。该蛋白的作用与经典途径中 C1 的作用相似。
种属

Mouse
表达系统

HEK293
标签

C-hFc
蛋白编号

P03953 (I26-S259)
基因 ID

11537  [NCBI]
蛋白结构
N-term
CFD (I26-S259)
Accession # P03953
hFc
C-term
同用名
Adipsin; C3 convertase activator; Complement factor D; CFD; PFD; DF; ADN; FACTOR D; AMBP-1; EC 3.4.21; EC 3.4.21.46
分子量

60-70 kDa
纯度

Greater than 95% as determined by Tris-Bis PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Complement Factor D/Adipsin Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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