Complement Factor D/Adipsin 蛋白, Rhesus macaque (HEK293, Fc)

Complement Factor D/Adipsin Protein is a member of the S1, or chymotrypsin, family of serine peptidases. Complement Factor D is expressed by adipose cells, plays an important role in both physiology and pathophysiology, where it plays a regulatory role in the complement system. Complement Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Complement Factor D/Adipsin Protein, Rhesus macaque (HEK293, Fc) is the recombinant mouse-derived Complement Factor D/Adipsin protein, expressed by HEK293, with C-hFc labeled tag.

补体因子 D (CFD) 也称为 Adipsin，是丝氨酸肽酶 S1 或胰凝乳蛋白酶家族的成员。CFD由脂肪细胞表达，在补体系统中发挥调节作用，在生理学和病理生理学中发挥着重要作用。补体系统被激活作为抵御入侵病原体的第一道防线，但也参与全身炎症、血栓形成甚至自身免疫性疾病。当因子 B 与因子 C3b 复合时，CFD 会裂解因子 B，从而在补体系统的替代途径中发挥至关重要的作用。这种裂解激活 C3bbb 复合物，将其转化为替代途径的 C3 转化酶。在与经典途径中的 C1 同源的过程中，CFD 协调补体级联的激活，有助于免疫反应和宿主防御机制。其裂解活性的特异性及其参与关键补体复合物形成的作用凸显了 CFD 在调节和放大针对病原体的免疫反应中的关键作用。此外，CFD 还可调节 I 型胶原蛋白表达和成纤维细胞迁移，以增强人体肌腱修复和愈合效果^[1][2]。

Mouse

HEK293

C-hFc

H9EXC1 (Q21-A253)

721138

Full Length of Mature Protein

Approximately 53 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution of Tris with Glycofe, Argofofe and NaCl, pH7.5 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Chen J, et al. Complement factor D regulates collagen type I expression and fibroblast migration to enhance human tendon repair and healing outcomes. Front Immunol. 2023 Sep 6;14:1225957.  [Content Brief]

  [2]. Forneris F, et al. Structures of C3b in complex with factors B and D give insight into complement convertase formation. Science. 2010 Dec 24;330(6012):1816-20.  [Content Brief]