Complement factor H/CFH 蛋白, Human (372a.a, HEK293, His)

Complement factor H/CFH proteins regulate complement activation and act as soluble inhibitors to prevent complement amplification on the cell surface. Complement factor H/CFH Protein, Human (372a.a, HEK293, His) is the recombinant human-derived Complement factor H/CFH protein, expressed by HEK293 , with C-His labeled tag. The total length of Complement factor H/CFH Protein, Human (372a.a, HEK293, His) is 372 a.a., with molecular weight of 55-60 kDa.

补体因子 H (CFH) 蛋白是一种糖蛋白，通过精细调节补体激活，在协调平衡免疫反应中发挥着关键作用。作为可溶性抑制剂，CFH 与自身标记（例如聚糖结构）结合，可有效防止细胞表面的补体激活和扩增。它的作用是加速补体旁路途径 (AP) C3 转化酶 C3bBb 的衰变，从而阻止更多 C3b（补体放大环中的关键成分）的局部形成。作为丝氨酸蛋白酶因子 I 的辅助因子，CFH 还可调节已沉积的 C3b 的蛋白水解降解。除了在补体调节中的作用外，CFH 还通过与特定受体的相互作用介导各种细胞反应。例如，它与 CR3/ITGAM 受体结合，促进人类中性粒细胞与多种病原体的粘附，导致随后的吞噬和破坏。值得注意的是，在微生物感染的情况下，CFH 与蚊子中肠中恶性疟原虫配子细胞的表面结合，提供针对替代补体途径介导的消除的保护。CFH 的多方面活性强调了其在免疫调节和防御病原体方面的关键作用。

Measured by its ability to bind biotinylated human DMP-1 in a functional ELISA.

Human

HEK293

C-His

P08603 (S860-R1231)

3075  [NCBI]

SIPLCVEKIPCSQPPQIEHGTINSSRSSQESYAHGTKLSYTCEGGFRISEENETTCYMGKWSSPPQCEGLPCKSPPEISHGVVAHMSDSYQYGEEVTYKCFEGFGIDGPAIAKCLGEKWSHPPSCIKTDCLSLPSFENAIPMGEKKDVYKAGEQVTYTCATYYKMDGASNVTCINSRWTGRPTCRDTSCVNPPTVQNAYIVSRQMSKYPSGERVRYQCRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGDITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVISREIMENYNIALRWTAKQKLYSRTGESVEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCAKR

55-60 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.