COMT 蛋白, Human (His)

Catechol-O-methyltransferase (COMT) plays a crucial role by catalyzing and inactivating the O-methylation of catecholamine neurotransmitters and catechol hormones. This enzyme activity is critical for regulating the biological half-life of key neuroactive compounds, including catecholamines. COMT Protein, Human (His) is the recombinant human-derived COMT protein, expressed by E. coli , with N-6*His labeled tag. The total length of COMT Protein, Human (His) is 220 a.a., with molecular weight of 25-28 kDa.

儿茶酚-O-甲基转移酶 (COMT) 是一种重要的酶，可催化儿茶酚胺神经递质和儿茶酚激素的 O-甲基化和随后的失活。通过介导甲基向这些分子的转移，COMT 在调节多巴胺、肾上腺素和去甲肾上腺素等神经递质的水平和活性方面发挥着关键作用。除了参与儿茶酚胺代谢外，COMT 还可以缩短特定神经活性药物的生物半衰期，包括左旋多巴、α-甲基多巴和异丙肾上腺素。这种酶活性对于调节针对神经和激素途径的治疗剂的药代动力学具有重要意义。COMT 在内源性神经递质和外源性药物中的调节作用凸显了其在神经传递和药物反应微调中的重要性，展示了其对生理和药理过程的广泛影响。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P21964 (G52-P271)

1312  [NCBI]

GDTKEQRILNHVLQHAEPGNAQSVLEAIDTYCEQKEWAMNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPGARLITIEINPDCAAITQRMVDFAGVKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLEYREVVDGLEKAIYKGPGSEAGP

25-28 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.